LOCUS       sequence-84208-e        2625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2625)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Calnexin-C-14
FEATURES             Location/Qualifiers
     source          1..2625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggaag tgctgggagc gcaagcggaa
      781 gcggaggctc cggtgaaggg aagtggttgc tgtgtatgtt actggtgctt ggaactgcta
      841 ttgttgaggc tcatgatgga catgatgatg atgtgattga tattgaggat gaccttgacg
      901 atgtcattga agaggtagaa gactcaaaac cagataccac tgctcctcct tcatctccca
      961 aggttactta caaagctcca gttccaacag gggaagtata ttttgctgat tcttttgaca
     1021 gaggaactct gtcagggtgg attttatcca aagccaagaa agacgatacc gatgatgaaa
     1081 ttgccaaata tgatggaaag tgggaggtag aggaaatgaa ggagtcaaag cttccaggtg
     1141 ataaaggact tgtgttgatg tctcgggcca agcatcatgc catctctgct aaactgaaca
     1201 agcccttcct gtttgacacc aagcctctca ttgttcagta tgaggttaat ttccaaaatg
     1261 gaatagaatg tggtggtgcc tatgtgaaac tgctttctaa aacaccagaa ctcaacctgg
     1321 atcagttcca tgacaagacc ccttatacga ttatgtttgg tccagataaa tgtggagagg
     1381 actataaact gcacttcatc ttccgacaca aaaaccccaa aacgggtatc tatgaagaaa
     1441 aacatgctaa gaggccagat gcagatctga agacctattt tactgataag aaaacacatc
     1501 tttacacact aatcttgaat ccagataata gttttgaaat actggttgac caatctgtgg
     1561 tgaatagtgg aaatctgctc aatgacatga ctcctcctgt aaatccttca cgtgaaattg
     1621 aggacccaga agaccggaag cccgaggatt gggatgaaag accaaaaatc ccagatccag
     1681 aagctgtcaa gccagatgac tgggatgaag atgcccctgc taagattcca gatgaagagg
     1741 ccacaaaacc cgaaggctgg ttagatgatg agcctgagta cgtacctgat ccagacgcag
     1801 agaaacctga ggattgggat gaagacatgg atggagaatg ggaggctcct cagattgcca
     1861 accctagatg tgagtcagct cctggatgtg gtgtctggca gcgacctgtg attgacaacc
     1921 ccaattataa aggcaaatgg aagcctccta tgattgacaa tcccagttac cagggaatct
     1981 ggaaacccag gaaaatacca aatccagatt tctttgaaga tctggaacct ttcagaatga
     2041 ctccttttag tgctattggt ttggagctgt ggtccatgac ctctgacatt ttttttgaca
     2101 actttatcat ttgtgctgat cgaagaatag ttgatgattg ggccaatgat ggatggggcc
     2161 tgaagaaagc tgctgatggg gctgctgagc caggcgttgt ggggcagatg aacgaggcag
     2221 ctgaagagcg cccgtggctg tgggtagtct atattctaac tgtagccctt cctgtgttcc
     2281 tggttatcct cttctgctgt tctggaaaga aacagaccag tggtatggag tataagaaaa
     2341 ctgatgcacc tcaaccggat gtgaaggaag aggaagaaga gaaggaagag gaaaaggaca
     2401 agggagatga ggaggaggaa ggagaagaga aacttgaaga gaaacagaaa agtgatgctg
     2461 aagaagatgg tggcactgtc agtcaagagg aggaagacag aaaacctaaa gcagaggagg
     2521 atgaaatttt gaacagatca ccaagaaaca gaaagccacg aagagagtga ggatccaccg
     2581 gatctagata actgatcata atcagccata ccacattgag acgtc
//
