LOCUS       sequence-84209-e        2616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2616)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1852..1861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1858..1860,1861..1863,1864..2577)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1903..1924)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2164..2183)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2511..2532
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagaccgcca tggaagggaa gtggttgctg
       61 tgtatgttac tggtgcttgg aactgctatt gttgaggctc atgatggaca tgatgatgat
      121 gtgattgata ttgaggatga ccttgacgat gtcattgaag aggtagaaga ctcaaaacca
      181 gataccactg ctcctccttc atctcccaag gttacttaca aagctccagt tccaacaggg
      241 gaagtatatt ttgctgattc ttttgacaga ggaactctgt cagggtggat tttatccaaa
      301 gccaagaaag acgataccga tgatgaaatt gccaaatatg atggaaagtg ggaggtagag
      361 gaaatgaagg agtcaaagct tccaggtgat aaaggacttg tgttgatgtc tcgggccaag
      421 catcatgcca tctctgctaa actgaacaag cccttcctgt ttgacaccaa gcctctcatt
      481 gttcagtatg aggttaattt ccaaaatgga atagaatgtg gtggtgccta tgtgaaactg
      541 ctttctaaaa caccagaact caacctggat cagttccatg acaagacccc ttatacgatt
      601 atgtttggtc cagataaatg tggagaggac tataaactgc acttcatctt ccgacacaaa
      661 aaccccaaaa cgggtatcta tgaagaaaaa catgctaaga ggccagatgc agatctgaag
      721 acctatttta ctgataagaa aacacatctt tacacactaa tcttgaatcc agataatagt
      781 tttgaaatac tggttgacca atctgtggtg aatagtggaa atctgctcaa tgacatgact
      841 cctcctgtaa atccttcacg tgaaattgag gacccagaag accggaagcc cgaggattgg
      901 gatgaaagac caaaaatccc agatccagaa gctgtcaagc cagatgactg ggatgaagat
      961 gcccctgcta agattccaga tgaagaggcc acaaaacccg aaggctggtt agatgatgag
     1021 cctgagtacg tacctgatcc agacgcagag aaacctgagg attgggatga agacatggat
     1081 ggagaatggg aggctcctca gattgccaac cctagatgtg agtcagctcc tggatgtggt
     1141 gtctggcagc gacctgtgat tgacaacccc aattataaag gcaaatggaa gcctcctatg
     1201 attgacaatc ccagttacca gggaatctgg aaacccagga aaataccaaa tccagatttc
     1261 tttgaagatc tggaaccttt cagaatgact ccttttagtg ctattggttt ggagctgtgg
     1321 tccatgacct ctgacatttt ttttgacaac tttatcattt gtgctgatcg aagaatagtt
     1381 gatgattggg ccaatgatgg atggggcctg aagaaagctg ctgatggggc tgctgagcca
     1441 ggcgttgtgg ggcagatgaa cgaggcagct gaagagcgcc cgtggctgtg ggtagtctat
     1501 attctaactg tagcccttcc tgtgttcctg gttatcctct tctgctgttc tggaaagaaa
     1561 cagaccagtg gtatggagta taagaaaact gatgcacctc aaccggatgt gaaggaagag
     1621 gaagaagaga aggaagagga aaaggacaag ggagatgagg aggaggaagg agaagagaaa
     1681 cttgaagaga aacagaaaag tgatgctgaa gaagatggtg gcactgtcag tcaagaggag
     1741 gaagacagaa aacctaaagc agaggaggat gaaattttga acagatcacc aagaaacaga
     1801 aagccacgaa gagagggggg tgcgtcaggc ggcagcggat ctggaccggt cgccaccatg
     1861 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1921 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1981 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2041 gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     2101 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2161 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2221 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2281 ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc
     2341 atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2401 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2461 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2521 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg
     2581 gccgcgactc tagatcataa tcagccatac cacatt
//
