LOCUS       sequence-8421-ex        5366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Gallardo T, Shirley L, John GB, Castrillon DH
  TITLE     Generation of a germ cell-specific mouse transgenic Cre line, 
            Vasa-Cre.
  JOURNAL   Genesis. 2007 Jun . 45(6):413-7.
  PUBMED    17551945
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Jun . 45(6):413-7."
COMMENT     Sequence Label: pVasa-Cre
FEATURES             Location/Qualifiers
     source          1..5366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             370..378
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tgtgccacca tgcctggccc agtttcttgc tttgtattat aaactttata gctggtaaga
       61 ctctggaccc acacgtgttt gatttgtctc ttctctcctg agacattttt tcccagtaac
      121 atttcctgaa ttttgtattt tattgtctct gcatctgtgc tgagacataa catcttctgt
      181 ttcttctatt tgccatgaac tgggagttaa gtctaagaat ttgattaaag ctgaagttga
      241 gtttgaaatc aaacctctac atgtagcgtt ttgtatttcc cactgtgata catcaaccag
      301 cacatggttc ttggaggtct ttgctttgtg gtataaagtt ggcccagtgt gtttaaatgt
      361 tgtcctcttt aaggaggtag ctaggtaaga tcacacgccg gttacctacc tccctactga
      421 tgcactaact ggtgtgtcta gcctagccta aggccctagg gtaccatgca tcccataata
      481 gcttatgtga ggcccaacac atttgtagat aacagcatca ggtcatagtg tcataaggtt
      541 ggacacccct ggcagagttt cctgtaccac actatcttca aaagagctaa agaagtaagg
      601 tggaaaaaaa caaacatcat agtgttctga ttttactgta acaggaaaag actcactgaa
      661 aagatgccaa ggcccagatt tcccctccca caattccaag cttcatggag aatgatacct
      721 ccactttggg gatggagaag gaattaaatc ctgaccacag acttgaagca gtaagaactt
      781 atcaccgtga tgtcagttct gggtggaacc ctatgcatcc cgtggccctg tacctgcaga
      841 tgctgctgct ggacctgtga tagatggcca gtactacagt taggaatctt tctgacaact
      901 gcagctttta gaatggaaat caaaactcca cctactggat ggcacaacat gaaagcaagg
      961 actgtgcctt gcagctcagg gccagcctta gtactagtct ctgagggacc gttgctacca
     1021 ccggttccca acctgtagca gcacaactat cctggaccag ggttcacgga tggagctcaa
     1081 gaccttgaca tctgtcacag ttatgctctg caccatgagg aagttgtctc agtttgcctt
     1141 actgccacag caccggctgt gggctcagca ttgtgacact cagagccaca gttctggagc
     1201 aggaaccagg gcaggtccct ctggtgattc tttctacagt tcacgaccag aggctgttgg
     1261 ctttggaagg cacctggagg tctgtgcaag ccccggacag aggcttgtgt agatgaagga
     1321 ccctttataa aaagctccct aattgagtct tgaaaaactc acccgctgag gatttaggag
     1381 aaacctaagc ttggatcgcc tcctaacact gccacaggta acctgaattt tggtgccata
     1441 tagtgttaga aactataggc tgagagagag agagaaaaaa aaaacattct tttttcaatt
     1501 tctgaaaaca aacaaaacca aaacaagcca tattatgaag accggaataa atacctaatc
     1561 ctttgtcctc gaatgacatc acacatcaat aagaaccaag gatgtttaag gaactatgac
     1621 ctcatctgac agtataaata taaaatgcca gaaaccgtca agatagtaac cagtgtgggt
     1681 ggatgctcat atgtgtactg tattagttac tttttcattg ttgtgataaa ataccacgac
     1741 taaagcctga cttataggag aaggaatttg tcctggcttg tgttttcaga gtgatatgac
     1801 tccatcatga cagggacgca tggaagccag caacaggtat gatgctgggg cagcaagctg
     1861 agagctcaca tccgccactg aaggcacaaa acagcaagtg tgagctggaa gtggtgtgag
     1921 gctatatact agcaaaggct gccctactga tacgctgtct ccaggaggac tgcattcccc
     1981 aagcttctct gatcatcaca accaactgag aactaaatgg tcaaatacct gggtcagtgg
     2041 ggatcatttc tcattcaaac ttccacagat aattaaaagc aaataaatga accaggttta
     2101 aggaaactta ctgaattcca atagaaaata tagaaataaa tcaatacttt agtagatata
     2161 attgactgag acatgggggc aaattttaaa aagttgaaat tcttgataat gaaggtatac
     2221 taaacaaaat aaaattgcat tagggaagca ttgatgttag agtaaatcaa gcagaagaga
     2281 gaatgagtga attccaacat aggccatttg aaaatgcaca gaagagaaaa agaattaaga
     2341 ggaagaaatg tactcatggt aaccttggag caacagtgaa agggtaagta ggttagtggg
     2401 gatatggacg ggacttgaga acaccaaaag agttaatcga tgcaactacg ttatgatcaa
     2461 acacagtcag gttccaggtt ctgcagtaaa ctgaatctcc agttttcacg ttatgtagat
     2521 ccatctccat gaatctggcc tggccttgtg aatttctggt aactgattag ttgaaagttg
     2581 caaatataat tgtttaactt cttatgctag aattcaagaa gccttgcaac tattaccctg
     2641 gtcctttgga gcgatgtccc tgacaagtgt gtccagttga aatgcagcat ccttacagag
     2701 tctgactgag attgtccaac aaccttcaga gcctaagtca gcctcttgga aactattcaa
     2761 ttgttctggc catcccagcc actagatgcc agagtagaga agtcaccttg gatgttaaac
     2821 aaactggtat tggaggttgg ggataaactc agtagtacag agcttgcctg gcacgctcaa
     2881 gtacctaggt tcaaactctg gcaatgccaa aaaagaaaaa agttgatagc aaactctcag
     2941 atgattcaag ttctaattag catttgaatg gaattgtatg agatggtctc ctgagccctc
     3001 tcaatccctg agagagaatt ttttttgtaa gtcaatatat tatgcaatag tttgttatat
     3061 agttgtactg gctaatttgg gtcaagctgg agttatcaca gagaaaggag cttcagttgg
     3121 ggaaatgcct ccatgagatc caactgtaag gcattttctc aattagtgat caagggggaa
     3181 aggccccttg tgggtaggac catctctggg ctggtagtct tggttctata agagagcagg
     3241 ctgagcaagc cagtaaggaa catccctcca tggcctctgc atcagctcct gctcctgacc
     3301 tgcttgagtt ccagtcctga cttcctttgg tgatgaacag caatgtggaa agtgtaagct
     3361 gaataaaccc tttcctcccc aatttgctca gtggtcatga tgttttgtcc tggaatagaa
     3421 accttgacta agacaatagt tataggtaac aagaagagag ctgtgaactc gtcatgtagt
     3481 taagtgtttg ctagatttcc tattgcatag ttcctatttt ctctttatat ttggaaaaaa
     3541 attgtggtaa cataccttag acacttattg ttgttctcct ccctgacctg cactcaatga
     3601 attctcagcc aaactctagt gatgtcctcc ccattcaaaa gcttcaacct tctagtcttt
     3661 attttaaaaa atgttattat tacatttatt tattctgtgg tgtgtgtgtg tgagagagag
     3721 gatgggagca ggtgtgtgtg tgtgtgtcgg tgtgtttgtt aaatcagaac atgagtgtgt
     3781 gtgtaagaca gagacggggt gagtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtcagtgg
     3841 ataagttgta gaagtcagtt ctttccttcc cttactcgga cctcagggat caaactcaag
     3901 ttctcaggtt gccaggtaca tgtcacctgt ctttgcttta ttattattta gttctggctt
     3961 ttgagcctaa gtgttatgtt acataggttg accttgaatt agctatgcac tgaggtcctg
     4021 atcctcctgc ctttatcttc caagtaatgg ttacaggaat gggccactgg acctagcaag
     4081 tgaacctacc tggtcactcc aaaaatgcac agcaaagaat atacgtttaa aaataggttc
     4141 attttaggaa gtttgtccac attttaaatg acagttctgt taaagtatac tgtgttttgt
     4201 cctttgttaa atgtgacttt taaaagcaat tcaccttaat agcctgggcg actacagtgc
     4261 tcactgtata aatgctagtg tgtttttggt gctgaaacag aaaggtggct ctagaaagct
     4321 ggagttcctc atctttaagt tccagactga gatatctaga acttcttcaa aaatccaggg
     4381 aaaggaaagc gatggaagtg aaaataaaaa caagaacatg ctttacatat atttgattgt
     4441 gatccctttg gcgggtacta ggaaaaccac ggatggaatt ttccttcttg aaagaggtga
     4501 ggagcaggca gaatgtgaac atctacttaa tgagctgaac tggccggtgc cctcagaatt
     4561 gtaaacaggt tcaccacaaa tccaggcctt ggcaaacaga ccaagtcttc ctctcttcgg
     4621 ttttcttttt acagactggc tttcttgaca acttcaagat ggagtctcat ccttgccctt
     4681 tttatggaga ggagaagcat tgcttctagt tggttttagt agaggagtga agtgcatttc
     4741 tcagatacaa agagagcact tgagacgttc agactcagaa tggccaagcc tggcactttg
     4801 ggaggtcaag aggaggctgg aacggctggg agagaaagca attagatgtt ccaccccttt
     4861 ggtttttctc cagacagggt ttctctgtgt agccctgact gtcctgaagt ttgctctgta
     4921 gaccaggctg gcctccaccc agggatttgc ctgcctctgc ctccccgagc cccagatttt
     4981 tatttttatt tatttatata tctattttaa cttttgaatg aacacaatgg aattgatgag
     5041 cccttggaga gagaaacggg atgtcgtgcg tggcagcccc ggggatcagc tcactcccac
     5101 aggcctcaca ggccatggag ccaagaggcc tccctgcctc ggcctcggcc tcggcctcaa
     5161 caaaggtgga gaacgcgcag gccgtccgtc catggggcgg gaagtcgcgc gccgcggccg
     5221 ctgattggct ggcgggcccg gtcgcctgat gctatttgtt gtccccgcgc caatgacgca
     5281 gtcggcgtcc cggcgtccgc ccgcacgtgc agccgtttaa gccgcgtcgg ccggccgcga
     5341 ggagcccggg gagcctggag cggaga
//
