LOCUS       sequence-84210-e        2100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2100)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Calreticulin-C-10
FEATURES             Location/Qualifiers
     source          1..2100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctg gtggcagtgg agggctgcta
      781 tccgtgccgc tgctgctcgg cctcctcggc ctggccgtcg ccgagcctgc cgtctacttc
      841 aaggagcagt ttctggacgg agacgggtgg acttcccgct ggatcgaatc caaacacaag
      901 tcagattttg gcaaattcgt tctcagttcc ggcaagttct acggtgacga ggagaaagat
      961 aaaggtttgc agacaagcca ggatgcacgc ttttatgctc tgtcggccag tttcgagcct
     1021 ttcagcaaca aaggccagac gctggtggtg cagttcacgg tgaaacatga gcagaacatc
     1081 gactgtgggg gcggctatgt gaagctgttt cctaatagtt tggaccagac agacatgcac
     1141 ggagactcag aatacaacat catgtttggt cccgacatct gtggccctgg caccaagaag
     1201 gttcatgtca tcttcaacta caagggcaag aacgtgctga tcaacaagga catccgttgc
     1261 aaggatgatg agtttacaca cctgtacaca ctgattgtgc ggccagacaa cacctatgag
     1321 gtgaagattg acaacagcca ggtggagtcc ggctccttgg aagacgattg ggacttcctg
     1381 ccacccaaga agataaagga tcctgatgct tcaaaaccgg aagactggga tgagcgggcc
     1441 aagatcgatg atcccacaga ctccaagcct gaggactggg acaagcccga gcatatccct
     1501 gaccctgatg ctaagaagcc cgaggactgg gatgaagaga tggacggaga gtgggaaccc
     1561 ccagtgattc agaaccctga gtacaagggt gagtggaagc cccggcagat cgacaaccca
     1621 gattacaagg gcacttggat ccacccagaa attgacaacc ccgagtattc tcccgatccc
     1681 agtatctatg cctatgataa ctttggcgtg ctgggcctgg acctctggca ggtcaagtct
     1741 ggcaccatct ttgacaactt cctcatcacc aacgatgagg catacgctga ggagtttggc
     1801 aacgagacgt ggggcgtaac aaaggcagca gagaaacaaa tgaaggacaa acaggacgag
     1861 gagcagaggc ttaaggagga ggaagaagac aagaaacgca aagaggagga ggaggcagag
     1921 gacaaggagg atgatgagga caaagatgag gatgaggagg atgaggagga caaggaggaa
     1981 gatgaggagg aagatgtccc cggccaggcc aaggacgagc tgtaggaatt ctgcagtcga
     2041 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
//
