LOCUS       sequence-84212-e        1836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1836)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..1836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1072..1081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1078..1080,1081..1083,1084..1797)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1123..1144)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1384..1403)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1731..1752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccagg
       61 atgtggtggt ccttgatccc tctctcttgc ctgctggcac tgaccagtgc ccatgacaag
      121 ccttcctttc acccactgtc ggacgacatg attaactata tcaacaaaca gaatacaaca
      181 tggcaggctg gacgcaactt ctacaatgtt gacataagct atctgaagaa gctgtgtgga
      241 actgtcctgg gtggacccaa gctgccggaa agggttgggt tcagcgagga cataaatcta
      301 cctgaatcct ttgatgcacg ggaacagtgg tccaattgcc cgaccatcgc acagatcaga
      361 gaccaggggt cctgtggctc ttgttgggca tttggggcag tggaagccat gtctgaccga
      421 atctgcattc acaccaatgg ccgagtcaat gtggaggtgt ctgctgagga cctgcttacc
      481 tgctgtggta tccagtgtgg ggatggctgt aatggtggct atccctctgg agcatggaac
      541 ttctggacta gaaaaggcct ggtttctggt ggagtataca attctcatat aggctgctta
      601 ccctacacca tccctccctg tgaacaccat gtcaatggct cccgtccccc atgcactgga
      661 gaaggagata ctcccaagtg caacaagatg tgtgaggctg gctactccac atcctacaag
      721 gaagataagc actatgggta cacttcctac agtgtgtctg acagcgagaa ggagatcatg
      781 gcggaaatct acaaaaatgg cccagtggag ggtgctttta ctgtgttttc tgacttcttg
      841 acttacaaat caggcgtata caagcatgaa gccggtgatg tgatgggagg ccatgccatc
      901 cgcattctgg gctggggaat agagaatgga gtaccctact ggctggtagc aaactcctgg
      961 aacgttgact ggggtgataa tggtttcttt aaaatcctca gaggagagaa ccactgtgga
     1021 attgaatcag aaatcgtggc tggaatccca cgcactcagg atccaccggt cgccaccatg
     1081 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1141 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1201 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1261 gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1321 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1381 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1441 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1501 ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc
     1561 atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1621 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1681 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1741 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg
     1801 gccgcgactc tagatcataa tcagccatac cacatt
//
