LOCUS       sequence-84213-e        1394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Hanson CA, Drake KR, Baird MA, Han B, Kraft LJ, Davidson MW, 
            Kenworthy AK
  TITLE     Overexpression of caveolin-1 is sufficient to phenocopy the behavior
            of a disease-associated mutant.
  JOURNAL   Traffic. 2013 Jun;14(6):663-77. doi: 10.1111/tra.12066. Epub 2013 
            Apr 7.
  PUBMED    23469926
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12066";
            journalName: "Traffic"; date: "2013-06"; volume: "14"; issue: "6"; 
            pages: "663-77"
COMMENT     Sequence Label: mEmerald-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggca gcagtggagg
      781 ttctgggggc aaatacgtag actcggaggg acatctctac accgttccca tccgggaaca
      841 gggcaacatc tacaagccca acaacaaggc catggcagac gagctgagcg agaagcaagt
      901 gtacgacgcg cacaccaagg agatcgacct ggtcaaccgc gaccctaaac acctcaacga
      961 tgacgtggtc aagattgact ttgaagatgt gattgcagaa ccagaaggga cacacagttt
     1021 tgacggcatt tggaaggcca gcttcaccac cttcactgtg acgaaatact ggttttaccg
     1081 cttgctgtct gccctctttg gcatcccgat ggcactcatc tggggcattt acttcgccat
     1141 tctctctttc ctgcacatct gggcagttgt accatgcatt aagagcttcc tgattgagat
     1201 tcagtgcatc agccgtgtct attccatcta cgtccacacc gtctgtgacc cactctttga
     1261 agctgttggg aaaatattca gcaatgtccg catcaacttg cagaaagaaa tataagaatt
     1321 ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca
     1381 gccataccac attt
//
