LOCUS       sequence-84214-e        1679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1679)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CCPB1-C-18
FEATURES             Location/Qualifiers
     source          1..1679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagag tccagtggat
      781 attcgtctcg gtcgggtgga agcagtgacc agcagctgga ttgtgccttg gatttgatga
      841 ggcgtctgcc tccgcagcag atagagaaga atctcagtga cctcattgac ttggtcccaa
      901 gcctctgtga agatctcctc tcctctgttg atcagccatt gaagattgca cgagataagg
      961 tggtaggaaa agactatcta ttgtgtgact acaacagaga tggagactcg tatagatcac
     1021 cgtggagtaa caagtatgat cctcccctgg aagacggtgc catgccatct gctcgcctgc
     1081 gcaagctgga ggtggaagcc aacaatgcct ttgaccagta tagagacttg tatttcgaag
     1141 gtggagtctc ctctgtctac ctctgggatc tggatcatgg ttttgccggt gtgatcctca
     1201 ttaagaaagc tggagatgga tcaaagaaga ttaagggatg ctgggattcc atccacgtgg
     1261 tggaggttca ggagaaatcc agcggccgta ctgctcatta caagctgacc tccacagtga
     1321 tgctgtggct gcagactaat aaaactggtt ctggtaccat gaaccttggc gggagcctca
     1381 ccagacagat ggagaaagat gagactgtga gcgactcttc tccgcacata gccaatattg
     1441 gacgcttggt agaggacatg gaaaacaaaa tcagaagtac actgaatgag atttattttg
     1501 gaaaaacaaa ggacatcgtg aatgggctga gatctattga tgctatcccc gacaaccaaa
     1561 agtataagca gttgcagagg gaactttctc aagtgttgac ccagcgccag atctacatcc
     1621 agcctgataa ctaagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatc
//
