LOCUS       sequence-84215-e        1536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1536)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CD9-10
FEATURES             Location/Qualifiers
     source          1..1536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(16..18,19..21,22..732)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(61..82)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(322..341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     669..690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
       61 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      121 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      181 gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac
      241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc
      481 gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc
      541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      601 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
      661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      721 gagctgtaca agtccggact cagatctcga gctcaagctt ccccggtcaa aggaggcacc
      781 aagtgcatca aatacctgct gttcggattt aacttcatct tctggcttgc cgggattgct
      841 gtccttgcca ttggactatg gctccgattc gactctcaga ccaagagcat cttcgagcaa
      901 gaaactaata ataataattc cagcttctac acaggagtct atattctgat cggagccggc
      961 gccctcatga tgctggtggg cttcctgggc tgctgcgggg ctgtgcagga gtcccagtgc
     1021 atgctgggac tgttcttcgg cttcctcttg gtgatattcg ccattgaaat agctgcggcc
     1081 atctggggat attcccacaa ggatgaggtg attaaggaag tccaggagtt ttacaaggac
     1141 acctacaaca agctgaaaac caaggatgag ccccagcggg aaacgctgaa agccatccac
     1201 tatgcgttga actgctgtgg tttggctggg ggcgtggaac agtttatctc agacatctgc
     1261 cccaagaagg acgtactcga aaccttcacc gtgaagtcct gtcctgatgc catcaaagag
     1321 gtcttcgaca ataaattcca catcatcggc gcagtgggca tcggcattgc cgtggtcatg
     1381 atatttggca tgatcttcag tatgatcttg tgctgtgcta tccgcaggaa ccgcgagatg
     1441 gtctagggat ccaagggcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     1501 atctagataa ctgatcataa tcagccatac cacatt
//
