LOCUS       sequence-84218-e        1586 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1586)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CD151-7
FEATURES             Location/Qualifiers
     source          1..1586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(19..21,22..24,25..735)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(325..344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
      241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggcggtatgg gtgagttcaa cgagaagaag
      781 acaacatgtg gcaccgtttg cctcaagtac ctgctgttta cctacaattg ctgcttctgg
      841 ctggctggcc tggctgtcat ggcagtgggc atctggacgc tggccctcaa gagtgactac
      901 atcagcctgc tggcctcagg cacctacctg gccacagcct acatcctggt ggtggcgggc
      961 actgtcgtca tggtgactgg ggtcttgggc tgctgcgcca ccttcaagga gcgtcggaac
     1021 ctgctgcgcc tgtacttcat cctgctcctc atcatctttc tgctggagat catcgctggt
     1081 atcctcgcct acgcctacta ccagcagctg aacacggagc tcaaggagaa cctgaaggac
     1141 accatgacca agcgctacca ccagccgggc catgaggctg tgaccagcgc tgtggaccag
     1201 ctgcagcagg agttccactg ctgtggcagc aacaactcac aggactggcg agacagtgag
     1261 tggatccgct cacaggaggc cggtggccgt gtggtcccag acagctgctg caagacggtg
     1321 gtggctcttt gtggacagcg agaccatgcc tccaacatct acaaggtgga gggcggctgc
     1381 atcaccaagt tggagacctt catccaggag cacctgaggg tcattggggc tgtggggatc
     1441 ggcattgcct gtgtgcaggt ctttggcatg atcttcacgt gctgcctgta caggagtctc
     1501 aagctggagc actactgaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     1561 atctagataa ctgatcataa tcagcc
//
