LOCUS       sequence-84225-e        1327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1327)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Centrin2-N-10
FEATURES             Location/Qualifiers
     source          1..1327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      566..575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(572..574,575..577,578..1291)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(617..638)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(878..897)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1225..1246
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc ctccaacttt aagaaggcaa acatggcatc
       61 aagttctcag cgaaaaagaa tgagccctaa gcctgagctt actgaagagc aaaagcagga
      121 gatccgggaa gcttttgatc ttttcgatgc ggatggaact ggcaccatag atgttaaaga
      181 actgaaggtg gcaatgaggg ccctgggctt tgaacccaag aaagaagaaa ttaagaaaat
      241 gataagtgaa attgataagg aagggacagg aaaaatgaac tttggtgact ttttaactgt
      301 gatgacccag aaaatgtctg agaaagatac taaagaagaa atcctgaaag ctttcaagct
      361 ctttgatgat gatgaaactg ggaagatttc gttcaaaaat ctgaaacgcg tggccaagga
      421 gttgggtgag aacctgactg atgaggagct gcaggaaatg attgatgaag ctgatcgaga
      481 tggagatgga gaggtcagtg agcaagagtt cctgcgcatc atgaaaaaga ccagcctcta
      541 ttcgggaagc acggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
      601 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      661 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      721 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
      781 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      841 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      901 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      961 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1021 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     1081 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1141 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     1201 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1261 cactctcggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
     1321 ataccac
//
