LOCUS       sequence-84226-e        1479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG
  TITLE     Time-lapse two-color 3D imaging of live cells with doubled 
            resolution using structured illumination.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5311-5. doi: 
            10.1073/pnas.1119262109. Epub 2012 Mar 19.
  PUBMED    22431626
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1119262109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-04-3- 3"; volume: "109"; issue: "14"; pages: "5311-5"
COMMENT     Sequence Label: mEmerald-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattctgca gtcgacatgg ctgatgactt tggcttcttc tcgtcgtcgg agagtggtgc
      841 cccggaggcg gcggaggagg acccggcggc cgccttcctg gcccagcagg agagcgagat
      901 tgcaggcata gagaacgacg agggcttcgg ggcacctgcc ggcagccatg cggcccccgc
      961 acagccgggc cccacgagtg gggctggttc tgaggacatg gggaccacag tcaatggaga
     1021 tgtgtttcag gaggccaacg gtcctgctga tggctacgca gccattgccc aggctgacag
     1081 gctgacccag gagcctgaga gcatccgcaa gtggcgagag gagcagagga aacggctgca
     1141 agagctggat gctgcatcta aggtcacgga acaggaatgg cgggagaagg ccaagaagga
     1201 cctggaggag tggaaccagc gccagagtga acaagtagag aagaacaaga tcaacaaccg
     1261 ggcatccgag gaggctttcg tgaaggaatc caaggaggag accccaggca cagagtggga
     1321 gaaggtggcc cagctatgtg acttcaaccc caagagcagc aagcagtgca aagatgtgtc
     1381 ccgcctgcgc tcggtgctca tgtccctgaa gcagacgcca ctgtcccgct agggatccac
     1441 cggatctaga taactgatca taatcagcca taccacatt
//
