LOCUS       sequence-84227-e        1466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1466)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Claudin7-C-12
FEATURES             Location/Qualifiers
     source          1..1466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctagcg gtggcagtgg
      781 aggtggcgcc aattcgggcc tgcagttgct gggcttctcc atggccctgc tgggctgggt
      841 gggtctggtg gcctgcaccg ccatcccgca gtggcagatg agctcctatg cgggtgacaa
      901 catcatcacg gcccaggcca tgtacaaggg gctgtggatg gactgcgtca cgcagagcac
      961 ggggatgatg agctgcaaaa tgtacgactc ggtgctcgcc ctgtccgcgg ccttgcaggc
     1021 cactcgagcc ctaatggtgg tctccctggt gctgggcttc ctggccatgt ttgtggccac
     1081 gatgggcatg aagtgcacgc gctgtggggg agacgacaaa gtgaagaagg cccgtatagc
     1141 catgggtgga ggcataattt tcatcgtggc aggtcttgcc accttggtag cttgctcctg
     1201 gtatggccat cagattgtca cagactttta taaccctttg atccctacca acattaagta
     1261 tgagtttggc cctgccatct ttattggctg ggcagggtct gccctagtca tcctgggagg
     1321 tgcactgctc tcctgttcct gtcctgggaa tgagagcaag gctgggtacc gtgcaccccg
     1381 ctcttaccct aagtccaact cttccaagga gtatgtgtga ggatccaccg gatctagata
     1441 actgatcata atcagccata ccacat
//
