LOCUS       sequence-84228-e        1453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1453)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Claudin7-N-12
FEATURES             Location/Qualifiers
     source          1..1453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      689..698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(695..697,698..700,701..1414)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(740..761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1001..1020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1348..1369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatggc caattcgggc ctgcagttgc tgggcttctc
       61 catggccctg ctgggctggg tgggtctggt ggcctgcacc gccatcccgc agtggcagat
      121 gagctcctat gcgggtgaca acatcatcac ggcccaggcc atgtacaagg ggctgtggat
      181 ggactgcgtc acgcagagca cggggatgat gagctgcaaa atgtacgact cggtgctcgc
      241 cctgtccgcg gccttgcagg ccactcgagc cctaatggtg gtctccctgg tgctgggctt
      301 cctggccatg tttgtggcca cgatgggcat gaagtgcacg cgctgtgggg gagacgacaa
      361 agtgaagaag gcccgtatag ccatgggtgg aggcataatt ttcatcgtgg caggtcttgc
      421 caccttggta gcttgctcct ggtatggcca tcagattgtc acagactttt ataacccttt
      481 gatccctacc aacattaagt atgagtttgg ccctgccatc tttattggct gggcagggtc
      541 tgccctagtc atcctgggag gtgcactgct ctcctgttcc tgtcctggga atgagagcaa
      601 ggctgggtac cgtgcacccc gctcttaccc taagtccaac tcttccaagg agtatgtggg
      661 cggtagctcc ggaggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      721 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      781 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      841 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      901 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      961 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1021 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1081 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1141 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
     1201 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1261 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1321 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1381 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
     1441 gccataccac att
//
