LOCUS       sequence-84229-e        5106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubule Binding , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5106)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CLIP170-C-18
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc
      241 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
      481 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
      541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattcaggg
      781 tctggaagtg gtagcagtat gctaaagcca agtgggctta aggcccccac caagatcctg
      841 aagcctggaa gcacagctct gaagacacct acggctgttg tagctccagt agaaaaaacc
      901 atatccagtg aaaaagcatc aagcactcca tcatctgaga ctcaggagga atttgtggat
      961 gactttcgag ttggggagcg agtttgggtg aatggaaata agcctggatt tatccagttt
     1021 cttggagaaa cccagtttgc accaggccag tgggctggaa ttgttttaga tgaacccata
     1081 ggcaagaacg atggttcggt ggcaggagtt cggtatttcc agtgtgaacc tttaaagggc
     1141 atatttaccc gaccttcaaa gttaacaagg aaggtgcaag cagaagatga agctaatggc
     1201 ctgcagacaa cgcccgcctc ccgagctact tcaccgctgt gcacttctac ggccagcatg
     1261 gtgtcttcct ccccctccac cccttcaaac atccctcaga aaccatcaca gccagcagca
     1321 aaggaacctt cagctacgcc tccgatcagc aaccttacaa aaactgccag tgaatctatc
     1381 tccaaccttt cagaggctgg ctcaatcaag aaaggagaaa gagagctcaa aatcggagac
     1441 agagtattgg ttggtggcac taaggctggt gtagtccggt ttcttgggga gaccgacttt
     1501 gccaaggggg agtggtgtgg cgtggagtta gatgagccac ttgggaagaa tgatggcgct
     1561 gttgctggaa caaggtattt tcagtgtcaa cccaaatatg gcttgttcgc tcctgtccac
     1621 aaagttacca agattggctt cccttccact acaccagcca aagccaaggc caacgcagtg
     1681 aggcgagtga tggcgaccac gtccgccagc ctgaagcgca gcccttctgc ctcttccctc
     1741 agctccatga gctcagtggc ctcctctgtg agcagcaggc ccagtcggac aggactattg
     1801 actgaaacct cctcccgtta cgccaggaag atctccggta ccactgccct ccaggaggcc
     1861 ctgaaggaga agcagcagca cattgagcag ctgctggcgg aacgggatct ggagagggcg
     1921 gaggtggcca aggccacgag ccacgtgggg gagatagagc aggagctagc tctggcccgg
     1981 gacggacatg accagcatgt cctggaattg gaagccaaaa tggaccagct gcgaacaatg
     2041 gtggaagctg ctgacaggga gaaggtggag cttctcaacc agcttgaaga ggagaaaagg
     2101 aaggttgagg accttcagtt ccgggttgaa gaagaatcaa ttaccaaagg tgatcttgag
     2161 acgcagacca aactggagca tgcccgcatt aaggagcttg aacagagcct gctctttgaa
     2221 aagaccaaag ctgacaaact ccagagggag ttagaagaca ctagggtggc tacagtttca
     2281 gaaaagtcac gtataatgga actggagaaa gacctagcat tgagagtaca ggaagtagct
     2341 gagctccgaa gaaggctaga gtccaataag cctgctgggg atgtggacat gtcactttcc
     2401 cttttgcaag agataagctc tttgcaagaa aagttagaag tcacccgtac tgaccaccag
     2461 agagaaataa cttctctgaa ggagcatttt ggagcccggg aagaaactca tcagaaggag
     2521 ataaaggctc tgtataccgc cacggaaaag ctttccaaag agaacgagtc attgaaaagc
     2581 aagctggagc atgccaacaa agagaactca gatgtgatag ctctatggaa gtccaaactg
     2641 gagactgcca tcgcatccca ccagcaggcg atggaagaac tgaaggtatc tttcagcaaa
     2701 gggcttggaa cagagacggc agaatttgct gaactaaaaa cacaaataga gaaaatgaga
     2761 ctagattacc aacacgaaat agaaaatttg cagaatcaac aagactctga acgggctgcc
     2821 catgctaaag agatggaagc cttgagggct aaactgatga aagttattaa agaaaaggaa
     2881 aacagtctgg aagccatcag gtcgaaactg gacaaagcag aagaccagca tctcgtagaa
     2941 atggaagaca cgttaaacaa attacaggaa gctgaaataa aggtaaagga gctagaggta
     3001 ctgcaagcca aatgcaatga acaaaccaag gttattgata attttacatc acagctcaag
     3061 gctactgaag aaaagctctt ggatcttgat gcacttcgga aagccagttc cgaaggtaaa
     3121 tcggaaatga agaaacttag acagcagctt gaggcagctg agaaacagat taaacattta
     3181 gagattgaaa agaatgctga aagtagcaag gctagtagca ttaccagaga gctccagggg
     3241 agagagctaa agcttactaa ccttcaggaa aatttgagtg aagtcagtca agtgaaagag
     3301 actttggaaa aagaacttca gattttgaaa gaaaagtttg ctgaagcttc agaggaggca
     3361 gtctctgttc agagaagtat gcaagaaact gtaaataagt tacaccaaaa ggaggaacag
     3421 tttaacatgc tgtcttctga cttggagaag ctgagagaaa acttagcaga tatggaggca
     3481 aaatttagag agaaagatga gagagaagag cagctgataa aggcaaagga aaaactggaa
     3541 aatgacattg cagaaataat gaagatgtca ggagataact cttctcagct gacaaaaatg
     3601 aacgatgaat tacgtctgaa agaaagagat gtagaagaat tacagctaaa acttacaaag
     3661 gctaatgaaa atgcaagttt tctgcaaaaa agtattgagg acatgactgt caaagctgaa
     3721 cagagccagc aagaagcagc taaaaagcat gaggaagaaa agaaagaatt ggagaggaaa
     3781 ttgtcggacc tggaaaagaa aatggaaaca agccacaacc agtgtcagga gctgaaagcc
     3841 aggtatgaga gagccacttc tgagacaaaa accaagcatg aagaaatcct acagaacctc
     3901 cagaagacgc tgctggacac agaggacaag ctgaagggcg cacgggagga gaacagtggc
     3961 ttgctgcagg agctggagga gctgagaaag caagccgaca aagccaaagc tgctcaaaca
     4021 gcggaagatg ccatgcagat aatggaacag atgaccaaag agaagactga gactctggcc
     4081 tccttggagg acaccaagca aacaaatgca aaactacaga atgaattgga cacacttaaa
     4141 gaaaacaact tgaaaaatgt ggaagagctg aacaaatcaa aagaactcct gactgtagag
     4201 aatcaaaaaa tggaagaatt taggaaagaa atagaaaccc taaagcaggc agcagctcag
     4261 aagtcccagc agctttcagc gttgcaagaa gagaacgtta aacttgctga ggagctgggg
     4321 agaagcaggg acgaagtcac aagtcatcaa aagctggaag aagaaagatc tgtgctcaat
     4381 aatcagttgt tagaaatgaa aaaaagagaa tccaagttca taaaagacgc agatgaagag
     4441 aaagcttcct tgcagaaatc catcagtata actagtgcct tactcacaga aaaggatgcc
     4501 gagctggaga aactgagaaa tgaggtcaca gtgctcaggg gagaaaacgc ctctgccaag
     4561 tccttgcatt cagttgttca gactctagag tctgataagg tgaagctcga gctcaaggta
     4621 aagaacttgg agcttcaact caaagaaaac aagaggcagc tcagcagctc ctcaggtaat
     4681 acagacactc aggcagacga ggatgaaaga gcccaggaga gtcagattga tttcctaaat
     4741 tcagtaatag tggaccttca aaggaagaat caagacctca agatgaaggt ggagatgatg
     4801 tcagaagcag ccctgaatgg gaacggggat gacctaaaca attatgacag tgatgatcag
     4861 gagaaacagt ccaagaagaa acctcgcctc ttctgtgaca tttgtgactg ctttgatctc
     4921 cacgacacag aggattgtcc tacccaggca cagatgtcag aggaccctcc ccattccaca
     4981 caccatggca gtcggggtga ggaacgccca tactgtgaaa tctgtgagat gtttggacac
     5041 tgggccacca actgcaatga cgacgaaacc ttctgaggat ccaccggatc tagataactg
     5101 atcata
//
