LOCUS       sequence-84230-e        2283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2283)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CNN-C-13
FEATURES             Location/Qualifiers
     source          1..2283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(39..41,42..44,45..755)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(84..105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(345..364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     692..713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aggtgacgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
       61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      121 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg
      241 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      301 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      481 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      541 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
      661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      721 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct ggctccagcg
      781 caggcagcgc atccaatagt aatcgaacgt cgtcttcgca caattcgaga aatctaaaga
      841 ttcttaatgc cagttttgac gttccacgac ctccaggcgg cggcaactcg cccttgccgt
      901 cacagggtcg ctctgtacgc gaattggagg agcagatgtc cgcgctgcgc aaggagaact
      961 tcaatctaaa gctgcgcatc tacttcctcg aggagggtca gccgggtgcc cgggcagaca
     1021 gctccacaga atccttaagc aaacagctca tcgatgccaa gatcgaaatc gcgacattga
     1081 gaaaaactgt cgatgtaaag atggagctgc tcaaggatgc cgctcgagcc atttctcatc
     1141 acgaggaatt gcagcgcaaa gcagacattg acagccaggc aataatcgac gagttgcaag
     1201 agcaaataca cgcctatcag atggcggagt ctggtggtca acctgtcgaa aatattgcca
     1261 aaaccaggaa aatgttgcgc cttgaatcgg aggtgcagag attggaggag gaactggtga
     1321 atatcgaagc tcgtaacgtt gcagcccgga acgagctgga attcatgttg gccgagcgcc
     1381 tagaatccct aacagcctgt gagggcaaga ttcaagagct ggccatcaag aattccgaac
     1441 tggtagagcg tcttgaaaag gaaacagcat ccgccgagtc atccaacgag gccatagact
     1501 ctcttaaggt tgaattagaa gcctgtagga aagagaatca ggatctggtg acctccattc
     1561 gaaccttaaa acatgatatg aagcgacaag tccgctcgat gaaggaggcc gccaatacga
     1621 tggacgtgca gcgtcagtcc atcctgctct tggaagccac aatcaagcgc aaggaaaagt
     1681 cgtgcggaag tatgcagaag aacgtgctaa actatgaggc actgatagcc aagttaaacg
     1741 ccgaactgga gactatgagg caacagaatg tttacttccg tgagctctcc gaaaacctac
     1801 aacagaagga ggtgcggcaa ctggatcgcg gcgtggccat cgtgcagccg atgcgcatga
     1861 cggcggatgc cgggcgtttt gtgtggcagt cagggaccat cgtcgcacaa gagcctttgc
     1921 cattggaaag gcagtcagct gcagcaacaa gcaacgtatc cgtgtccgca gtacgtttat
     1981 catcagggcc accaccagac cagacatacc cagttaatgg ccatgaccgc gccaagtacg
     2041 gccttctaca actattcgct gccaagcttg actggatttc ggcagttcct ttggcgctgg
     2101 ggcacttagc cctcaagatc atcctcttgg ctatgcggtt gcactcctgc ctccagaatg
     2161 tccatatccc tcttagggtg tcctttgagc tggggcattc catgccgttg gctggcattc
     2221 tgtactctat ttgaggatcc accggatcta gataactgat cataatcagc cataccacat
     2281 tgt
//
