LOCUS       sequence-84231-e        2276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2276)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-CNN-N-18
FEATURES             Location/Qualifiers
     source          1..2276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1513..1522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1519..1521,1522..1524,1525..2238)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1564..1585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1825..1844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2172..2193
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgaat agtaatcgaa cgtcgtcttc gcacaattcg
       61 agaaatctaa agattcttaa tgccagtttt gacgttccac gacctccagg cggcggcaac
      121 tcgcccttgc cgtcacaggg tcgctctgta cgcgaattgg aggagcagat gtccgcgctg
      181 cgcaaggaga acttcaatct aaagctgcgc atctacttcc tcgaggaggg tcagccgggt
      241 gcccgggcag acagctccac agaatcctta agcaaacagc tcatcgatgc caagatcgaa
      301 atcgcgacat tgagaaaaac tgtcgatgta aagatggagc tgctcaagga tgccgctcga
      361 gccatttctc atcacgagga attgcagcgc aaagcagaca ttgacagcca ggcaataatc
      421 gacgagttgc aagagcaaat acacgcctat cagatggcgg agtctggtgg tcaacctgtc
      481 gaaaatattg ccaaaaccag gaaaatgttg cgccttgaat cggaggtgca gagattggag
      541 gaggaactgg tgaatatcga agctcgtaac gttgcagccc ggaacgagct ggaattcatg
      601 ttggccgagc gcctagaatc cctaacagcc tgtgagggca agattcaaga gctggccatc
      661 aagaattccg aactggtaga gcgtcttgaa aaggaaacag catccgccga gtcatccaac
      721 gaggccatag actctcttaa ggttgaatta gaagcctgta ggaaagagaa tcaggatctg
      781 gtgacctcca ttcgaacctt aaaacatgat atgaagcgac aagtccgctc gatgaaggag
      841 gccgccaata cgatggacgt gcagcgtcag tccatcctgc tcttggaagc cacaatcaag
      901 cgcaaggaaa agtcgtgcgg aagtatgcag aagaacgtgc taaactatga ggcactgata
      961 gccaagttaa acgccgaact ggagactatg aggcaacaga atgtttactt ccgtgagctc
     1021 tccgaaaacc tacaacagaa ggaggtgcgg caactggatc gcggcgtggc catcgtgcag
     1081 ccgatgcgca tgacggcgga tgccgggcgt tttgtgtggc agtcagggac catcgtcgca
     1141 caagagcctt tgccattgga aaggcagtca gctgcagcaa caagcaacgt atccgtgtcc
     1201 gcagtacgtt tatcatcagg gccaccacca gaccagacat acccagttaa tggccatgac
     1261 cgcgccaagt acggccttct acaactattc gctgccaagc ttgactggat ttcggcagtt
     1321 cctttggcgc tggggcactt agccctcaag atcatcctct tggctatgcg gttgcactcc
     1381 tgcctccaga atgtccatat ccctcttagg gtgtcctttg agctggggca ttccatgccg
     1441 ttggctggca ttctgtactc tattagcagc ggtggagcaa gcgcagccag tggtagcgcg
     1501 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1561 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1621 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1681 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
     1741 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1861 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1921 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     1981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     2041 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2101 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     2161 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2221 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacat
//
