LOCUS       sequence-84234-e        2295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2295)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Coronin1B-C-10
FEATURES             Location/Qualifiers
     source          1..2295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctagcg gtggcagtgg
      781 ctccttccgc aaagtggtcc ggcagagcaa attccggcat gtgttcgggc agccggtcaa
      841 gaacgaccag tgctatgagg acattcgcgt gtcccgtgtt acctgggaca gcaccttctg
      901 cgccgtcaac cccaagttcc tggcggtgat tgtggaggcc agtggagggg gtgcctttct
      961 ggtgctcccc ctaagcaaga cgggccgcat tgacaaggcc tacccgacgg tgtgtgggca
     1021 cacgggacct gtcctggaca tcgactggtg tcctcacaac gacgaagtca tagccagcgg
     1081 ctcggaggac tgcacggtca tggtgtggca gatcccagag aacgggctga cctccccgct
     1141 gacagagccg gtggtggtac tggaggggca caccaagcga gtgggcatca tcgcctggca
     1201 ccccacggcc cgaaacgtgc tgctcagtgc aggctgcgac aacgtggtac tcatctggaa
     1261 tgtgggcaca gcggaggagc tgtaccgcct ggacagcctg caccctgacc tcatctacaa
     1321 tgtcagctgg aaccacaatg gcagcctgtt ttgctcagca tgcaaggaca agagcgtgcg
     1381 catcatcgac ccccgtcggg gcaccctggt ggcagagcgg gagaaggctc atgagggggc
     1441 ccggcccatg cgggccatct tcctggcaga tggcaaggtg ttcaccacag gcttcagccg
     1501 aatgagcgag cggcagctgg cgctctggga cccagaaaac ctcgaggaac ccatggccct
     1561 gcaggaactg gactcgagca acggggccct gctgcccttc tacgaccccg acaccagtgt
     1621 ggtctacgtc tgcggcaagg gtgactccag catccggtac tttgagatca cagaggagcc
     1681 tccctacatc cacttcctga acacgttcac cagcaaggag ccgcagcggg gtatgggcag
     1741 catgcccaag cggggcctgg aggtcagcaa gtgcgagatc gcccggttct acaaactgca
     1801 tgagcgcaag tgtgagccca tcgtcatgac tgtgccaaga aagtcggacc tcttccagga
     1861 tgatctgtac cccgacacag ccgggcccga ggcagccctg gaggctgagg agtgggtgag
     1921 cgggcgggat gccgacccga tcctcatctc actgcgggag gcctacgtgc ccagcaagca
     1981 gcgggacctg aagatcagcc ggcgcaacgt gttgtctgac agccggcccg ccatggcccc
     2041 gggctcctcc cacctagggg cccccgcctc caccaccact gctgctgatg ccacccccag
     2101 cggcagcctg gccagagccg gggaggctgg gaagctggag gaggtgatgc aggagctgcg
     2161 ggccctgagg gcgctggtca aggagcaggg cgaccgcatc tgccgcctgg aggagcagct
     2221 gggccgcatg gagaacgggg atgcgtaggg atccaccgga tctagataac tgatcataat
     2281 cagccatacc acatt
//
