LOCUS       sequence-84235-e        2272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2272)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Coronin1B-N-10
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1515..1524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1521..1523,1524..1526,1527..2240)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1566..1587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1827..1846)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2174..2195
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgtcct tccgcaaagt ggtccggcag agcaaattcc
       61 ggcatgtgtt cgggcagccg gtcaagaacg accagtgcta tgaggacatt cgcgtgtccc
      121 gtgttacctg ggacagcacc ttctgcgccg tcaaccccaa gttcctggcg gtgattgtgg
      181 aggccagtgg agggggtgcc tttctggtgc tccccctaag caagacgggc cgcattgaca
      241 aggcctaccc gacggtgtgt gggcacacgg gacctgtcct ggacatcgac tggtgtcctc
      301 acaacgacga agtcatagcc agcggctcgg aggactgcac ggtcatggtg tggcagatcc
      361 cagagaacgg gctgacctcc ccgctgacag agccggtggt ggtactggag gggcacacca
      421 agcgagtggg catcatcgcc tggcacccca cggcccgaaa cgtgctgctc agtgcaggct
      481 gcgacaacgt ggtactcatc tggaatgtgg gcacagcgga ggagctgtac cgcctggaca
      541 gcctgcaccc tgacctcatc tacaatgtca gctggaacca caatggcagc ctgttttgct
      601 cagcatgcaa ggacaagagc gtgcgcatca tcgacccccg tcggggcacc ctggtggcag
      661 agcgggagaa ggctcatgag ggggcccggc ccatgcgggc catcttcctg gcagatggca
      721 aggtgttcac cacaggcttc agccgaatga gcgagcggca gctggcgctc tgggacccag
      781 aaaacctcga ggaacccatg gccctgcagg aactggactc gagcaacggg gccctgctgc
      841 ccttctacga ccccgacacc agtgtggtct acgtctgcgg caagggtgac tccagcatcc
      901 ggtactttga gatcacagag gagcctccct acatccactt cctgaacacg ttcaccagca
      961 aggagccgca gcggggtatg ggcagcatgc ccaagcgggg cctggaggtc agcaagtgcg
     1021 agatcgcccg gttctacaaa ctgcatgagc gcaagtgtga gcccatcgtc atgactgtgc
     1081 caagaaagtc ggacctcttc caggatgatc tgtaccccga cacagccggg cccgaggcag
     1141 ccctggaggc tgaggagtgg gtgagcgggc gggatgccga cccgatcctc atctcactgc
     1201 gggaggccta cgtgcccagc aagcagcggg acctgaagat cagccggcgc aacgtgttgt
     1261 ctgacagccg gcccgccatg gccccgggct cctcccacct aggggccccc gcctccacca
     1321 ccactgctgc tgatgccacc cccagcggca gcctggccag agccggggag gctgggaagc
     1381 tggaggaggt gatgcaggag ctgcgggccc tgagggcgct ggtcaaggag cagggcgacc
     1441 gcatctgccg cctggaggag cagctgggcc gcatggagaa cggggatgcg ggcggtagcg
     1501 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1561 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1621 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1681 tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc
     1741 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1801 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1861 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1921 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     1981 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     2041 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2101 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     2161 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2221 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca ta
//
