LOCUS       sequence-84238-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Fort AG, Murray JW, Dandachi N, Davidson MW, Dermietzel R, Wolkoff 
            AW, Spray DC
  TITLE     In vitro motility of liver connexin vesicles along microtubules 
            utilizes kinesin motors.
  JOURNAL   J Biol Chem. 2011 Jul 1;286(26):22875-85. doi: 
            10.1074/jbc.M111.219709. Epub 2011 May 2.
  PUBMED    21536677
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.219709"; journalName: "J Biol Chem"; date: 
            "2011-07-1- 1"; volume: "286"; issue: "26"; pages: "22875-85"
COMMENT     Sequence Label: mEmerald-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(982..984,985..987,988..1701)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1027..1048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1288..1307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1635..1656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc ctctgggaaa
       61 gggcagcagc agccaggtgt ggcagtgaca gggaggtgtg aatgaggcag gatgaactgg
      121 acaggtttgt acaccttgct cagtggcgtg aaccggcatt ctactgccat tggccgagta
      181 tggctctcgg tcatcttcat cttcagaatc atggtgctgg tggtggctgc agagagtgtg
      241 tggggtgatg agaaatcttc cttcatctgc aacacactcc agcctggctg caacagcgtt
      301 tgctatgacc aattcttccc catctcccat gtgcggctgt ggtccctgca gctcatccta
      361 gtttccaccc cagctctcct cgtggccatg cacgtggctc accagcaaca catagagaag
      421 aaaatgctac ggcttgaggg ccatggggac cccctacacc tggaggaggt gaagaggcac
      481 aaggtccaca tctcagggac actgtggtgg acctatgtca tcagcgtggt gttccggctg
      541 ttgtttgagg ccgtcttcat gtatgtcttt tatctgctct accctggcta tgccatggtg
      601 cggctggtca agtgcgacgt ctacccctgc cccaacacag tggactgctt cgtgtcccgc
      661 cccaccgaga aaaccgtctt caccgtcttc atgctagctg cctctggcat ctgcatcatc
      721 ctcaatgtgg ccgaggtggt gtacctcatc atccgggcct gtgcccgccg agcccagcgc
      781 cgctccaatc caccttcccg caagggctcg ggcttcggcc accgcctctc acctgaatac
      841 aagcagaatg agatcaacaa gctgctgagt gagcaggatg gctccctgaa agacatactg
      901 cgccgcagcc ctggcaccgg ggctgggctg gctgaaaaga gcgaccgctg ctcggcctgc
      961 gcggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1021 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1081 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1141 ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca gtgcttcgcc
     1201 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1261 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1321 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1381 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc
     1441 accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag
     1501 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1561 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     1621 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1681 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
     1741 tgtagaggtt ttacttgctt taaaaaacct cccac
//
