LOCUS       sequence-84240-e        1600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1600)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Dectin1A-C-10
FEATURES             Location/Qualifiers
     source          1..1600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggcag cagtggaggt
      781 gaatatcatc ctgatttaga aaatttggat gaagatggat atactcaatt acacttcgac
      841 tctcaaagca ataccaggat agctgttgtt tcagagaaag gatcgtgtgc tgcatctcct
      901 ccttggcgcc tcattgctgt aattttggga atcctatgct tggtaatact ggtgatagct
      961 gtggtcctgg gtaccatggc tatttggaga tccaattcag gaagcaacac attggagaat
     1021 ggctactttc tatcaagaaa taaagagaac cacagtcaac ccacacaatc atctttagaa
     1081 gacagtgtga ctcctaccaa agctgtcaaa accacagggg ttctttccag cccttgtcct
     1141 cctaattgga ttatatatga gaagagctgt tatctattca gcatgtcact aaattcctgg
     1201 gatggaagta aaagacaatg ctggcaactg ggctctaatc tcctaaagat agacagctca
     1261 aatgaattgg gatttatagt aaaacaagtg tcttcccaac ctgataattc attttggata
     1321 ggcctttctc ggccccagac tgaagtacca tggctctggg aggatggatc aacattctct
     1381 tctaacttat ttcagatcag aaccacagct acccaagaaa acccatctcc aaattgtgta
     1441 tggattcacg tgtcagtcat ttatgaccaa ctgtgtagtg tgccctcata tagtatttgt
     1501 gagaagaagt tttcaatgta agaattctgc agtcgacggt accgcgggcc cgggatccac
     1561 cggatctaga taactgatca taatcagcca taccacattt
//
