LOCUS       sequence-84241-e        1549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1549)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Dectin1A-N-10
FEATURES             Location/Qualifiers
     source          1..1549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(790..792,793..795,796..1509)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(835..856)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1096..1115)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1443..1464
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgccaccat ggaatatcat cctgatttag aaaatttgga tgaagatgga
       61 tatactcaat tacacttcga ctctcaaagc aataccagga tagctgttgt ttcagagaaa
      121 ggatcgtgtg ctgcatctcc tccttggcgc ctcattgctg taattttggg aatcctatgc
      181 ttggtaatac tggtgatagc tgtggtcctg ggtaccatgg ctatttggag atccaattca
      241 ggaagcaaca cattggagaa tggctacttt ctatcaagaa ataaagagaa ccacagtcaa
      301 cccacacaat catctttaga agacagtgtg actcctacca aagctgtcaa aaccacaggg
      361 gttctttcca gcccttgtcc tcctaattgg attatatatg agaagagctg ttatctattc
      421 agcatgtcac taaattcctg ggatggaagt aaaagacaat gctggcaact gggctctaat
      481 ctcctaaaga tagacagctc aaatgaattg ggatttatag taaaacaagt gtcttcccaa
      541 cctgataatt cattttggat aggcctttct cggccccaga ctgaagtacc atggctctgg
      601 gaggatggat caacattctc ttctaactta tttcagatca gaaccacagc tacccaagaa
      661 aacccatctc caaattgtgt atggattcac gtgtcagtca tttatgacca actgtgtagt
      721 gtgccctcat atagtatttg tgagaagaag ttttcaatga gctccggagg ggatccaccg
      781 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      901 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      961 tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac
     1021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1081 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1201 ctggggcaca agctggagta caactacaac agccacaagg tctatatcac cgccgacaag
     1261 cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg
     1321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1381 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1501 tacaagtaag cggccgcgac tctagatcat aatcagccat accacattt
//
