LOCUS       sequence-84243-e        2253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2253)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Desmin-N-18
FEATURES             Location/Qualifiers
     source          1..2253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1297..1308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      1486..1495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1492..1494,1495..1497,1498..2211)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1537..1558)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1798..1817)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2145..2166
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgccaccatg agccaggcct actcgtccag ccagcgcgtg
       61 tcctcctacc gccgcacctt cggcggggcc ccgggcttcc cgctcggctc cccgctgagc
      121 tcgcccgtgt tcccgcgggc gggtttcggc tctaagggct cctccagctc ggtgacgtcc
      181 cgcgtgtacc aggtgtcgcg cacgtcgggc ggggccgggg gcctggggtc gctgcgggcc
      241 agccggctgg ggaccacccg cacgccctcc tcctacggcg caggcgagct gctggacttc
      301 tcactggccg acgcggtgaa ccaggagttt ctgaccacgc gcaccaacga gaaggtggag
      361 ctgcaggagc tcaatgaccg cttcgccaac tacatcgaga aggtgcgctt cctggagcag
      421 cagaacgcgg cgctcgccgc cgaagtgaac cggctcaagg gccgcgagcc gacgcgagtg
      481 gccgagctct acgaggagga gctgcgggag ctgcggcgcc aggtggaggt gctcactaac
      541 cagcgcgcgc gcgtcgacgt cgagcgcgac aacctgctcg acgacctgca gcggctcaag
      601 gccaagctgc aggaggagat tcagttgaag gaagaagcag agaacaattt ggctgccttc
      661 cgagcggacg tggatgcagc tactctagct cgcattgacc tggagcgcag aattgaatct
      721 ctcaacgagg agatcgcgtt ccttaagaaa gtgcatgaag aggagatccg tgagttgcag
      781 gctcagcttc aggaacagca ggtccaggtg gagatggaca tgtctaagcc agacctcact
      841 gccgccctca gggatatccg ggctcagtat gagaccatcg cggctaagaa catttctgaa
      901 gctgaggagt ggtacaagtc gaaggtgtca gacctgaccc aggcagccaa caagaacaac
      961 gacgccctgc gccaggccaa gcaggagatg atggaatacc gacaccagat ccagtcctac
     1021 acctgcgaga ttgacgccct caagggcact aacgattccc tgatgaggca gatgcgggaa
     1081 ttggaggacc gatttgccag tgaggccagt ggctaccagg acaacattgc acgcctggag
     1141 gaggaaatcc ggcacctcaa ggatgagatg gcccgccatc tgcgcgagta ccaggacctg
     1201 ctcaacgtga agatggccct ggatgtggag attgccacct accggaagct gctggaggga
     1261 gaggagagcc ggatcaatct ccccatccag acctactctg ccctcaattt ccgagaaacc
     1321 agccctgagc aaaggggttc tgaggtccat accaagaaga cggtgatgat caagaccatc
     1381 gagacacggg atggggaggt cgtcagtgag gccacacagc agcagcatga agtgctcagc
     1441 agcggtggag caagcgcagc cagtggtagc gcggatccac cggtcgccac catggtgagc
     1501 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1561 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1621 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1681 accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1741 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1801 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1861 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1921 tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag
     1981 gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2041 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2101 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2161 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg
     2221 actctagatc ataatcagcc ataccacatt gta
//
