LOCUS       sequence-84247-e        4181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4181)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Desmoglein2-N-18
FEATURES             Location/Qualifiers
     source          1..4181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3430..3439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3436..3438,3439..3441,3442..4155)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3481..3502)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3742..3761)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4089..4110
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgt cgccaccatg gcgcggagcc cgggacgcgc gtacgccctg
       61 ctgcttctcc tgatctgctt taacgttgga agtggacttc acttacaggt cttaagcaca
      121 agaaatgaaa ataagctgct tcctaaacat cctcatttag tgcggcaaaa gcgcgcctgg
      181 atcaccgccc ccgtggctct tcgggaggga gaggatctgt ccaagaagaa tccaattgcc
      241 aagatacatt ctgatcttgc agaagaaaga ggactcaaaa ttacttacaa atacactgga
      301 aaagggatta cagagccacc ttttggtata tttgtcttta acaaagatac tggagaactg
      361 aatgttacca gcattcttga tcgagaagaa acaccatttt ttctgctaac aggttacgct
      421 ttggatgcaa gaggaaacaa tgtagagaaa cccttagagc tacgcattaa ggttcttgat
      481 atcaatgaca acgaaccagt gttcacacag gatgtctttg ttgggtctgt tgaagagttg
      541 agtgcagcac atactcttgt gatgaaaatc aatgcaacag atgcagatga gcccaatacc
      601 ctgaactcga aaatttccta tagaatcgta tctctggagc ctgcttatcc tccagtgttc
      661 tacctaaata aagatacagg agagatttat acaaccagtg ttaccttgga cagagaggaa
      721 cacagcagct acactttgac agtagaagca agagatggca atggagaagt tacagacaaa
      781 cctgtaaaac aagctcaagt tcagattcgt attttggatg tcaatgacaa tatacctgta
      841 gtagaaaata aagtgcttga agggatggtt gaagaaaatc aagtcaacgt agaagttacg
      901 cgcataaaag tgttcgatgc agatgaaata ggttctgata attggctggc aaattttaca
      961 tttgcatcag gaaatgaagg aggttatttc cacatagaaa cagatgctca aactaacgaa
     1021 ggaattgtga cccttattaa ggaagtagat tatgaagaaa tgaagaatct tgacttcagt
     1081 gttattgtcg ctaataaagc agcttttcac aagtcgatta ggagtaaata caagcctaca
     1141 cccattccca tcaaggtcaa agtgaaaaat gtgaaagaag gcattcattt taaaagcagc
     1201 gtcatctcaa tttatgttag cgagagcatg gatagatcaa gcaaaggcca aataattgga
     1261 aattttcaag catttgatga ggacactgga ctaccagccc atgcaagata tgtaaaatta
     1321 gaagatagag ataattggat ctctgtggat tctgtcacat ctgaaattaa acttgcaaaa
     1381 cttcctgatt ttgaatctag atatgttcaa aatggcacat acactgtaaa gattgtggcc
     1441 atatcagaag attatcctag aaaaaccatc actggcacag tccttatcaa tgttgaagac
     1501 atcaacgaca actgtcccac actgatagag cctgtgcaga caatctgtca cgatgcagag
     1561 tatgtgaatg ttactgcaga ggacctggat ggacacccaa acagtggccc tttcagtttc
     1621 tccgtcattg acaaaccacc tggcatggca gaaaaatgga aaatagcacg ccaagaaagt
     1681 accagtgtgc tgctgcaaca aagtgagaaa aagctaggga gaagtgaaat tcagttcctg
     1741 atttcagaca atcagggttt tagttgtcct gaaaagcagg tccttacact cacagtttgt
     1801 gagtgtctgc atggcagcgg ctgcagggaa gcacagcatg actcctatgt gggcctggga
     1861 cccgcagcaa ttgcgctcat gattttggcc tttctgctcc tgctattggt gccactttta
     1921 ctgctgatgt gccattgcgg aaagggcgcc aaaggcttta cccccatacc tggcaccata
     1981 gagatgctgc atccttggaa taatgaagga gcaccacctg aagacaaggt ggtgccatca
     2041 tttctgccag tggatcaagg gggcagtcta gtaggaagaa atggagtagg aggtatggcc
     2101 aaggaagcca cgatgaaagg aagtagctct gcttccattg tcaaagggca acatgagatg
     2161 tccgagatgg atggaaggtg ggaagaacac agaagcctgc tttctggtag agctacccag
     2221 tttacagggg ccacaggcgc tatcatgacc actgaaacca cgaagaccgc aagggccaca
     2281 ggggcttcca gagacatggc cggagctcag gcagctgctg ttgcactgaa cgaagagttc
     2341 ttaagaaatt atttcactga taaagcggcc tcttacactg aggaagatga aaatcacaca
     2401 gccaaagatt gccttctggt ttattctcag gaagaaactg aatcgctgaa tgcttctatt
     2461 ggttgttgca gttttattga aggagagcta gatgaccgct tcttagatga tttgggactt
     2521 aaattcaaga cactagctga agtttgcctg ggtcaaaaaa tagatataaa taaggaaatt
     2581 gagcagagac aaaaacctgc cacagaaaca agtatgaaca cagcttcaca ttcactctgt
     2641 gagcaaacta tggttaattc agagaatacc tactcctctg gcagtagctt cccagttcca
     2701 aaatctttgc aagaagccaa tgcagagaaa gtaactcagg aaatagtcac tgaaaggtct
     2761 gtgtcttcta ggcaggcgca aaaggtagct acacctcttc ctgacccaat ggcttctaga
     2821 aatgtgatag caacagaaac ttcctatgtc acagggtcca ctatgccacc aaccactgtg
     2881 atcctgggtc ctagccagcc acagagcctt attgtgacag agagggtgta tgctccagct
     2941 tctaccttgg tagatcagcc ttatgctaat gaaggtacag ttgtggtcac tgaaagagta
     3001 atacagcctc atgggggtgg atcgaatcct ctggaaggca ctcagcatct tcaagatgta
     3061 ccttacgtca tggtgaggga aagagagagc ttccttgccc ccagctcagg tgtgcagcct
     3121 actctggcca tgcctaatat agcagtagga cagaatgtga cagtgacaga aagagttcta
     3181 gcacctgctt ccactctgca atccagttac cagattccca ctgaaaattc tatgacggct
     3241 aggaacacca cggtgtctgg agctggagtc cctggccctc tgccagattt tggtttagag
     3301 gaatctggtc attctaattc taccataacc acatcttcca ccagagttac caagcatagc
     3361 actgtacagc attcttactc cagcagcggt ggagcaagcg cagccagtgg tagcgcggat
     3421 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3481 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3541 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     3601 gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac
     3661 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3721 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3781 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3841 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc
     3901 gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc
     3961 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4021 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     4081 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4141 gagctgtaca agtaagcggc cgcgactcta gatcataatc a
//
