LOCUS       sequence-84253-e        1664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1664)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-EB1-N-18
FEATURES             Location/Qualifiers
     source          1..1664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      887..896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(893..895,896..898,899..1612)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(938..959)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1199..1218)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1546..1567
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acatgatgtg accgtcagat ccgctagcgc caccatggca gtgaacgtat actcaacgtc
       61 agtgaccagt gataacctaa gtcgacatga catgctggcc tggatcaatg agtctctgca
      121 gttgaatctg acaaagatcg aacagttgtg ctcaggggct gcgtattgtc agtttatgga
      181 catgctgttc cctggctcca ttgccttgaa gaaagtgaaa ttccaagcta agctagaaca
      241 cgagtacatc cagaacttca aaatattaca agcaggtttt aagagaatgg gtgttgacaa
      301 aataattcct gtggacaaat tagtaaaagg aaagtttcag gacaattttg aattcgttca
      361 gtggttcaag aagttcttcg atgcaaacta tgatggaaaa gactatgacc ctgtggctgc
      421 tagacaaggt caagaaactg cagtggctcc ttcccttgtt gctccagctc tgaataaacc
      481 gaagaaacct ctcacttcta gcagtgcagc tccccagagg cccatctcaa cacagagaac
      541 cgctgcggct cctaaggctg gccctggtgt ggtgcgaaag aaccctggtg tgggcaacgg
      601 agacgacgag gcagctgagt tgatgcagca ggtcaacgta ttgaaactta ctgttgaaga
      661 cttggagaaa gagagggatt tctacttcgg aaagctacgg aacattgaat tgatttgcca
      721 ggagaacgag ggggaaaacg accctgtatt gcagaggatt gtagacattc tgtatgccac
      781 agatgaaggc tttgtgatac ctgatgaagg gggcccacag gaggagcaag aagagtatag
      841 cagcggtgga gcaagcgcag ccagtggtag cgcggatcca ccggtcgcca ccatggtgag
      901 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      961 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1021 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1081 caccttgacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     1141 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1201 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1261 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1321 gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa
     1381 ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1441 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1501 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1561 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc
     1621 gactctagat cataatcagc cataccacat tgtagaggta ttcc
//
