LOCUS       sequence-84254-e        1671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, 
            Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, 
            Bewersdorf J
  TITLE     Video-rate nanoscopy using sCMOS camera-specific single-molecule 
            localization algorithms.
  JOURNAL   Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 
            2013 May 26.
  PUBMED    23708387
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2488"; journalName: "Nat Methods"; date: "2013-07"; 
            volume: "10"; issue: "7"; pages: "653-8"
COMMENT     Sequence Label: mEmerald-EB3-7
FEATURES             Location/Qualifiers
     source          1..1671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      908..917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(914..916,917..919,920..1633)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(959..980)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1220..1239)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1567..1588
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggactcagat ctcgagctca agcttcgaat tctgcagtcg actgccacca tggccgtcaa
       61 tgtgtactcc acatctgtga ccagtgaaaa tctgagtcgc catgatatgc ttgcatgggt
      121 caacgactcc ctgcacctca actataccaa gatagaacag ctttgttcag gggcagccta
      181 ctgccagttc atggacatgc tcttccccgg ctgtgtgcac ttgaggaaag tgaagttcca
      241 ggccaaacta gagcatgaat acatccacaa cttcaaggtg ctgcaagcag ctttcaagaa
      301 gatgggtgtt gacaaaatca ttcctgtaga gaaattagtg aaaggaaaat tccaagataa
      361 ttttgagttt attcagtggt ttaagaaatt ctttgacgca aactatgatg gaaaggatta
      421 caaccctctg ctggcgcggc agggccagga cgtagcgcca cctcctaacc caggtgatca
      481 gatcttcaac aaatccaaga aactcattgg cacagcagtt ccacagagga cgtcccccac
      541 aggcccaaaa aacatgcaga cctctggccg gctgagcaat gtggcccccc cctgcattct
      601 ccggaagaat cctccatcag cccgaaatgg cggccatgag actgatgccc aaattcttga
      661 actcaaccaa cagctggtgg acttgaagct gacagtggat gggctggaga aggaacgtga
      721 cttctacttc agcaaacttc gtgacatcga gctcatctgc caggagcatg aaagtgaaaa
      781 cagccctgtt atctcaggca tcattggcat cctctatgcc acagaggaag gattcgcacc
      841 ccctgaggac gatgagattg aagagcatca acaagaagac caggacgagt acctggatcc
      901 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      961 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1021 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1081 gccctggccc accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc
     1141 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1201 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1261 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1321 catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga
     1381 caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag
     1441 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1501 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     1561 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1621 gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca t
//
