LOCUS       sequence-84255-e        1700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1700)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-EB3-C-20
FEATURES             Location/Qualifiers
     source          1..1700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc
      781 agcgcatccg gcggaagcgg aagcggggga gccgtcaatg tgtactccac atctgtgacc
      841 agtgaaaatc tgagtcgcca tgatatgctt gcatgggtca acgactccct gcacctcaac
      901 tataccaaga tagaacagct ttgttcaggg gcagcctact gccagttcat ggacatgctc
      961 ttccccggct gtgtgcactt gaggaaagtg aagttccagg ccaaactaga gcatgaatac
     1021 atccacaact tcaaggtgct gcaagcagct ttcaagaaga tgggtgttga caaaatcatt
     1081 cctgtagaga aattagtgaa aggaaaattc caagataatt ttgagtttat tcagtggttt
     1141 aagaaattct ttgacgcaaa ctatgatgga aaggattaca accctctgct ggcgcggcag
     1201 ggccaggacg tagcgccacc tcctaaccca ggtgatcaga tattcaacaa atccaagaaa
     1261 ctcattggca cagcagttcc acagaggacg tcccccacag gcccaaaaaa catgcagacc
     1321 tctggccggc tgagcaatgt ggcccccccc tgcattctcc ggaagaatcc tccatcagcc
     1381 cgaaatggcg gccatgagac tgatgcccaa attcttgaac tcaaccaaca gctggtggac
     1441 ttgaagctga cagtggatgg gctggagaag gaacgtgact tctacttcag caaacttcgt
     1501 gacatcgagc tcatctgcca ggagcatgaa agtgaaaaca gccctgttat ctcaggcatc
     1561 attggcatcc tctatgccac agaggaagga ttcgcacccc ctgaggacga tgagattgaa
     1621 gagcatcaac aagaagacca ggacgagtac taaggatcca ccggatctag ataactgatc
     1681 ataatcagcc ataccacatt
//
