LOCUS       sequence-84256-e        3422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoplasm, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3422)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-EIF2C3-C-18
FEATURES             Location/Qualifiers
     source          1..3422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ggtctggcgg
      781 tagttcggga tcttcgtcca gcggagaaat cggctccgca ggacccgctg gggcccagcc
      841 cctactcatg gtgcccagaa gacctggcta tggcaccatg ggcaaaccca ttaaactgct
      901 ggctaactgt tttcaagttg aaatcccaaa gattgatgtc tacctctatg aggtagatat
      961 taaaccagac aagtgtccta ggagagtgaa cagggaggtg gttgactcaa tggttcagca
     1021 ttttaaagta actatatttg gagaccgtag accagtttat gatggaaaaa gaagtcttta
     1081 caccgccaat ccacttcctg tggcaactac aggggtagat ttagacgtta ctttacctgg
     1141 ggaaggtgga aaagatcgac ctttcaaggt gtcaatcaaa tttgtctctc gggtgagttg
     1201 gcacctactg catgaagtac tgacaggacg gaccttgcct gagccactgg aattagacaa
     1261 gccaatcagc actaaccctg tccatgccgt tgatgtggtg ctacgacatc tgccctccat
     1321 gaaatacaca cctgtggggc gttcattttt ctccgctcca gaaggatatg accaccctct
     1381 gggagggggc agggaagtgt ggtttggatt ccatcagtct gttcggcctg ccatgtggaa
     1441 aatgatgctt aatatcgatg tttctgccac tgccttctac aaagcacaac ctgtaattca
     1501 gttcatgtgt gaagttcttg atattcataa tattgatgag caaccaagac ctctgactga
     1561 ttctcatcgg gtaaaattca ccaaagagat aaaaggtttg aaggttgaag tgactcattg
     1621 tggaacaatg agacggaaat accgtgtttg taatgtaaca aggaggcctg ccagtcatca
     1681 aacctttcct ttacagttag aaaacggcca aactgtggag agaacagtag cgcagtattt
     1741 cagagaaaag tatactcttc agctgaagta cccgcacctt ccctgtctgc aagtcgggca
     1801 ggaacagaaa cacacctacc tgccactaga agtctgtaat attgtggcag ggcaacgatg
     1861 tatcaagaag ctaacagaca atcagacttc cactatgatc aaggcaacag caagatctgc
     1921 accagataga caagaggaaa ttagcagatt ggtaagaagt gcaaattatg aaacagatcc
     1981 atttgttcag gagtttcaat ttaaagttcg ggatgaaatg gctcatgtaa ctggacgcgt
     2041 acttccagca cctatgctcc agtatggagg acggaatcgg acagtagcaa caccgagcca
     2101 tggagtatgg gacatgcgag ggaaacaatt ccacacagga gttgaaatca aaatgtgggc
     2161 tatcgcttgt tttgccacac agaggcagtg cagagaagaa atattgaagg gtttcacaga
     2221 ccagctgcgt aagatttcta aggatgcagg gatgcccatc cagggccagc catgcttctg
     2281 caaatatgca cagggggcag acagcgtaga gcccatgttc cggcatctca agaacacata
     2341 ttctggccta cagcttatta tcgtcatcct gccggggaag acaccagtgt atgcggaagt
     2401 gaaacgtgta ggagacacac ttttgggtat ggctacacaa tgtgttcaag tcaagaatgt
     2461 aataaaaaca tctcctcaaa ctctgtcaaa cttgtgccta aagataaatg ttaaactcgg
     2521 agggatcaat aatattcttg tacctcatca aagaccttct gtgttccagc aaccagtgat
     2581 ctttttggga gccgatgtca ctcatccacc tgctggtgat ggaaagaagc cttctattgc
     2641 tgctgttgta ggtagtatgg atgcacaccc aagcagatac tgtgccacag taagagttca
     2701 gagaccccga caggagatca tccaggactt ggcctccatg gtccgggaac ttcttattca
     2761 attttataag tcaactcggt tcaagcctac tcgtatcatc ttttatcggg atggtgtttc
     2821 agaggggcag tttaggcagg tattatatta tgaactacta gcaattcgag aagcctgcat
     2881 cagtttggag aaagactatc aacctggaat aacctacatt gtagttcaga agagacatca
     2941 cactcgatta ttttgtgctg ataggacaga aagggttgga agaagtggca atatcccagc
     3001 tggaacaaca gttgatacag acattacaca cccatatgag ttcgattttt acctctgtag
     3061 ccatgctgga atacagggta ccagtcgtcc ttcacactat catgttttat gggatgataa
     3121 ctgctttact gcagatgaac ttcagctgct aacttaccag ctctgccaca cttacgtacg
     3181 ctgtacacga tctgtttcta tacctgcacc agcgtattat gctcacctgg tagcatttag
     3241 agccagatat catcttgtgg acaaagaaca tgacagtgct gaaggaagtc acgtttcagg
     3301 acaaagcaat gggcgagatc cacaagctct tgccaaggct gtacagattc accaagatac
     3361 cttacgcaca atgtacttcg cttaaggatc ccccggatct agataactga tcataatcag
     3421 cc
//
