LOCUS       sequence-84257-e        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/Endoplasmic Reticulum, Excitation: 487, 
            Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ELP-1-25
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      734..743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(740..742,743..745,746..1459)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(785..806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1046..1065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1393..1414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgccgcca ccatggccat gaacattttc cggctgactg gggacctgtc
       61 ccacctggcg gccatcgtca tcctgctgct gaagatctgg aagacgcgct cctgcgccgg
      121 tatttctggg aaaagccagc ttctgtttgc actggtcttc acaactcgtt acctggatct
      181 ttttacttca tttatttcat tgtataacac atctatgaag gttatctacc ttgcctgctc
      241 ctatgccaca gtgtacctga tctacctgaa atttaaggca acctacgatg gaaatcatga
      301 taccttccga gtggagtttc tggtggtccc tgtgggaggc ctctcatttt tagttaatca
      361 cgatttctct cctcttgaga tcctctggac cttctccatc tacctggagt ccgtggctat
      421 ccttccgcag ctgtttatga tcagcaagac tggggaggcc gagaccatca ccacccacta
      481 cctgttcttc ctgggcctct atcgtgcttt gtatcttgtc aactggatct ggcgcttcta
      541 ctttgagggc ttctttgacc tcattgctgt ggtggccggc gtagtccaga ccatcctata
      601 ctgtgacttc ttctacttgt acattacaaa agtactcaag ggaaagaagc tcagtttgcc
      661 agcaggcggt gggggggagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg
      721 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      781 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      841 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      901 gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
      961 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1021 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1081 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1141 cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg tctatatcac
     1201 cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga
     1261 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1321 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     1381 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1441 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat accaca
//
