LOCUS       sequence-84258-e        1472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1472)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Endo-14
FEATURES             Location/Qualifiers
     source          1..1472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             790..819
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga tcagatctcg agctcaagct tgatatcgaa
      781 ttccccatgg agcagaagct gatctccgag gaggacctga accggaagaa actggtggtt
      841 gttggtgatg gagcctgtgg aaagacatgc ttgctcatag tcttcagcaa ggacgagttc
      901 ccagaggtgt atgtgcccac agtgtttgag aactatgtgg cagatatcga ggtggatgga
      961 aagcaggtag agttggcttt gtgggacaca gctggccagg aggactacga ccgcctgcgg
     1021 ccgctctcct acccggacac cgacgtcatt ctcatgtgct tctcggtgga cagcccggac
     1081 tcgctggaga acatccccga gaagtgggtc cccgaggtga agcacttctg tcccaatgtg
     1141 cccatcatcc tggtggccaa caaaaaagac ctgcgcagcg acgagcatgt ccgcacagag
     1201 ctggcccgca tgaagcagga acccgttcgc acggatgacg gccgcgccat ggccgtgcgc
     1261 atccaagcct acgactacct cgagtgctct gccaagacca aggaaggcgt gcgcgaggtc
     1321 ttcgagacgg ccacgcgcgc cgcgctgcag aagcgctacg gctcccagaa cggctgcatc
     1381 aactgctgca aggtgctatg agggccgcgc ccgtcgcgcc tgcccctgcc ggggatccac
     1441 cggatctaga taactgatca taatcagcca ta
//
