LOCUS       sequence-84261-e        3860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3860)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Espin-like-C-18
FEATURES             Location/Qualifiers
     source          1..3860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattcc
      781 ggtggcagcg gcgggagtga ggcgcagcag gcactggtgg cctccaagga tggggatatg
      841 gccaccttgg aacggctatt tgaggctggt gccctaagac cagacatcac tgatgatctg
      901 ggggctggcc tggtgcatca tgccacccgg gctggtcacc tggactgtgt caagttcctg
      961 gtacagagag ccaagctgcc aggcaaccag caggcacaca acggggccac accagtgcac
     1021 gatgcagcag ccacgggcaa cctggcagag ctgtgctggc tggtccgtga tgcgggctgc
     1081 gggctgcagg accaagatgc ctcaggtgtc tctccgctgc acctggctgc ccggtttgga
     1141 cacccagccc tggtggagtg gttactccgt gagggccatg cagccacact ggagacgctg
     1201 gaaggggcct taccactgca ccacgctgct gtaagtggtg atctgacctg tctgaaactc
     1261 ctgacagcgg cccacagcag tggtgtgaac cagcggacat gcagtggtgc ctctccactc
     1321 tacctggcct gccaagaggg ccacctgcat ctggcacagt tcctggtgaa agactgcggg
     1381 gccgacgtgc gcctgagagc cctggatggc atgagctcat tgcacgctgc ggctgcccac
     1441 ggccactact ctctagttgt ctggctggtg acattcacag acatcggcct gacagcacgg
     1501 gataatgaag gagccactgc cctgcacttt gcagctcggg ggggccacac acctattctc
     1561 gaccggctcc tgctcatggg tgcccccatc atgagagact cttggggagg gactcccctg
     1621 catgatgcag ccgagaatgg acacatggag tgctgccaga ctctgctctc ccaccacgta
     1681 gaccccttcc tgcgggatga agatgggtat acagcgatag acctggcaga gtaccatgga
     1741 caccaggatt gtgcccagtt cctgcgggaa atgtctcggc cggtgcgggt cctgatgaca
     1801 cccccacccc caccatttcc acctcctcca ctgctggctg caaaactctc cctggaagag
     1861 gaaagaagag gggactcggg actcaaaagc ccctcatctg caactctcag cccagtctgg
     1921 cctgctcagc ctgtcccaag ggaaccgatg gcctgcacgg cccctctgag ggtcaccacc
     1981 ccagatgctc ttcaggggcc tgaggtggaa gcccgggact cccgtgctgg cctagccact
     2041 ctgcagctgg atggcttgcc tgcgggagat cttgacatgc tggtgcccac acaggacgag
     2101 cgtggccggc ccatccctga gtggaagcgg caggtgatgg tgcggaagct acaggctcgc
     2161 ctgggtgcag accatcctcc agaagaccag gaccagagtc agaggcagga ctcagggccc
     2221 accgctgcag aacaggcaac ttggagatat tcacagacgc accaggccat cctgggcccc
     2281 tttggagagc tgctgacgga ggatgatctt gtatacctgg agaagcagat caacgacctg
     2341 cagctccggc gacgctgcca ggagtatgag agtgagttgg gccggctggc agcccagctg
     2401 caggccctcc tgcccgagcc cctggtcagc atcaccgtca acagccactt tctgccccgg
     2461 gctcccggcc tggaagacga ggaagctcca gtcctggcga ctgaactcga agcctccgag
     2521 gagccgggga aggccgagcc cagagggcag ccgctgccct tctggtgcag ccacatcggt
     2581 cgcctggtgc gcagcatgtc cctgctgctg aagggtatga acgggctggc acagggtgag
     2641 gagaaaccac cctcaccggt cccgcaggac ctaggcaagg agaccattgc cggcccctcc
     2701 cggagcgagg cccagcgcga gatccaagag tgcggagtgt cagttcggac attgcggggg
     2761 aacttcgagt ttgcccctga tctgccctgc gccttgaact cgggtccctg tgagctgggg
     2821 gtgcggcctg gccagtgtct gagaggttgc tggtcggcgc cgccacagcc ccgaggcgac
     2881 gcgatgggtg gggagcccgg gccaggcgac acagaggagg ccagcgactc gggcatcagc
     2941 tgcgaagagg ccccgtcaga ggccggcgcg ggtccagggc tggacctggc cagcttgcgc
     3001 aaggagcgca tcgtcatgct cttcctcggc cattggaaga agtcagccta cacgcccgcg
     3061 ctccggacgg cagcctgcag gaccctggag gctcagcgtg cacgatccag gggcccagag
     3121 gccgcgggta gcccccggcc gtcctccccg cagcccagcg atggccctcg gcttggccac
     3181 ctgtggcagc agcgtggcat catcacccac ctgctgggca cctggaaagc catcatggcg
     3241 cacgtgcccg cccggcagct gcggaggctg agccgcaggg agcgtgggcc cctgtctccg
     3301 gagcagttct tgccgcacgt ggacggggcc cccgtgccct acaacagcct ctcgctggac
     3361 ctcttcatgc ttggctactt ccagctcctg gagtgcgacc tgccagccga ggagcgcaag
     3421 atgcgccacc tgctgtgctt cgaggttttc gagcatctgg gcgcacacgg ctgggaggct
     3481 gtgcgagcct tccataaggc agtgaccgac gaggtggccg cgggtcgccg cgcctggacc
     3541 gacggctttg aggacatcaa agcccgcttc ttcggctcca gccagggtcc cccctgggac
     3601 gtggagccag gtcgcaagct gggcctgaca ccactcgggt ccttgcccca tgcttccctt
     3661 cctggcagcg gccctgaacc tgcggtgccg ccgaggctgg ggtctgattc ccaggggagc
     3721 agcttcaaca gcggagacat ctgtggctac atcgaccgca gcttcgcatt ctggaaggag
     3781 aaagaggctg agatgtttaa ctttggagaa tgagtcgacg gtaccgcggg cccgggatcc
     3841 accggatcta gataactgat
//
