LOCUS       sequence-84262-e        3854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3854)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Espin-like-N-18
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3095..3104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3101..3103,3104..3106,3107..3820)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3146..3167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3407..3426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3754..3775
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatctcgagc tcaagcttcg aattcgccac catggaggcg cagcaggcac tggtggcctc
       61 caaggatggg gatatggcca ccttggaacg gctatttgag gctggtgccc taagaccaga
      121 catcactgat gatctggggg ctggcctggt gcatcatgcc acccgggctg gtcacctgga
      181 ctgtgtcaag ttcctggtac agagagccaa gctgccaggc aaccagcagg cacacaacgg
      241 ggccacacca gtgcacgatg cagcagccac gggcaacctg gcagagctgt gctggctggt
      301 ccgtgatgcg ggctgcgggc tgcaggacca agatgcctca ggtgtctctc cgctgcacct
      361 ggctgcccgg tttggacacc cagccctggt ggagtggtta ctccgtgagg gccatgcagc
      421 cacactggag acgctggaag gggccttacc actgcaccac gctgctgtaa gtggtgatct
      481 gacctgtctg aaactcctga cagcggccca cagcagtggt gtgaaccagc ggacatgcag
      541 tggtgcctct ccactctacc tggcctgcca agagggccac ctgcatctgg cacagttcct
      601 ggtgaaagac tgcggggccg acgtgcgcct gagagccctg gatggcatga gctcattgca
      661 cgctgcggct gcccacggcc actactctct agttgtctgg ctggtgacat tcacagacat
      721 cggcctgaca gcacgggata atgaaggagc cactgccctg cactttgcag ctcggggggg
      781 ccacacacct attctcgacc ggctcctgct catgggtgcc cccatcatga gagactcttg
      841 gggagggact cccctgcatg atgcagccga gaatggacac atggagtgct gccagactct
      901 gctctcccac cacgtagacc ccttcctgcg ggatgaagat gggtatacag cgatagacct
      961 ggcagagtac catggacacc aggattgtgc ccagttcctg cgggaaatgt ctcggccggt
     1021 gcgggtcctg atgacacccc cacccccacc atttccacct cctccactgc tggctgcaaa
     1081 actctccctg gaagaggaaa gaagagggga ctcgggactc aaaagcccct catctgcaac
     1141 tctcagccca gtctggcctg ctcagcctgt cccaagggaa ccgatggcct gcacggcccc
     1201 tctgagggtc accaccccag atgctcttca ggggcctgag gtggaagccc gggactcccg
     1261 tgctggccta gccactctgc agctggatgg cttgcctgcg ggagatcttg acatgctggt
     1321 gcccacacag gacgagcgtg gccggcccat ccctgagtgg aagcggcagg tgatggtgcg
     1381 gaagctacag gctcgcctgg gtgcagacca tcctccagaa gaccaggacc agagtcagag
     1441 gcaggactca gggcccaccg ctgcagaaca ggcaacttgg agatattcac agacgcacca
     1501 ggccatcctg ggcccctttg gagagctgct gacggaggat gatcttgtat acctggagaa
     1561 gcagatcaac gacctgcagc tccggcgacg ctgccaggag tatgagagtg agttgggccg
     1621 gctggcagcc cagctgcagg ccctcctgcc cgagcccctg gtcagcatca ccgtcaacag
     1681 ccactttctg ccccgggctc ccggcctgga agacgaggaa gctccagtcc tggcgactga
     1741 actcgaagcc tccgaggagc cggggaaggc cgagcccaga gggcagccgc tgcccttctg
     1801 gtgcagccac atcggtcgcc tggtgcgcag catgtccctg ctgctgaagg gtatgaacgg
     1861 gctggcacag ggtgaggaga aaccaccctc accggtcccg caggacctag gcaaggagac
     1921 cattgccggc ccctcccgga gcgaggccca gcgcgagatc caagagtgcg gagtgtcagt
     1981 tcggacattg cgggggaact tcgagtttgc ccctgatctg ccctgcgcct tgaactcggg
     2041 tccctgtgag ctgggggtgc ggcctggcca gtgtctgaga ggttgctggt cggcgccgcc
     2101 acagccccga ggcgacgcga tgggtgggga gcccgggcca ggcgacacag aggaggccag
     2161 cgactcgggc atcagctgcg aagaggcccc gtcagaggcc ggcgcgggtc cagggctgga
     2221 cctggccagc ttgcgcaagg agcgcatcgt catgctcttc ctcggccatt ggaagaagtc
     2281 agcctacacg cccgcgctcc ggacggcagc ctgcaggacc ctggaggctc agcgtgcacg
     2341 atccaggggc ccagaggccg cgggtagccc ccggccgtcc tccccgcagc ccagcgatgg
     2401 ccctcggctt ggccacctgt ggcagcagcg tggcatcatc acccacctgc tgggcacctg
     2461 gaaagccatc atggcgcacg tgcccgcccg gcagctgcgg aggctgagcc gcagggagcg
     2521 tgggcccctg tctccggagc agttcttgcc gcacgtggac ggggcccccg tgccctacaa
     2581 cagcctctcg ctggacctct tcatgcttgg ctacttccag ctcctggagt gcgacctgcc
     2641 agccgaggag cgcaagatgc gccacctgct gtgcttcgag gttttcgagc atctgggcgc
     2701 acacggctgg gaggctgtgc gagccttcca taaggcagtg accgacgagg tggccgcggg
     2761 tcgccgcgcc tggaccgacg gctttgagga catcaaagcc cgcttcttcg gctccagcca
     2821 gggtcccccc tgggacgtgg agccaggtcg caagctgggc ctgacaccac tcgggtcctt
     2881 gccccatgct tcccttcctg gcagcggccc tgaacctgcg gtgccgccga ggctggggtc
     2941 tgattcccag gggagcagct tcaacagcgg agacatctgt ggctacatcg accgcagctt
     3001 cgcattctgg aaggagaaag aggctgagat gtttaacttt ggagaaagcg gcggaagtgg
     3061 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
     3121 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3181 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3241 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta
     3301 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3361 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3421 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3481 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3541 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3601 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3661 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     3721 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3781 cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     3841 taatcagcca tacc
//
