LOCUS       sequence-84263-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions/Membrane, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Ezrin-C-14
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagg aagcggcgga
      781 tctgggtccg gtccgaaacc aatcaatgtc cgagttacca ccatggatgc agagctggag
      841 tttgcaatcc agccaaatac aactggaaaa cagctttttg atcaggtggt aaagactatc
      901 ggcctccggg aagtgtggta ctttggcctc cactatgtgg ataataaagg atttcctacc
      961 tggctgaagc tggataagaa ggtgtctgcc caggaggtca ggaaggagaa tcccctccag
     1021 ttcaagttcc gggccaagtt ctaccctgaa gatgtggctg aggagctcat ccaggacatc
     1081 acccagaaac ttttcttcct ccaagtgaag gaaggaatcc ttagcgatga gatctactgc
     1141 ccccctgaga ctgccgtgct cttggggtcc tacgctgtgc aggccaagtt tggggactac
     1201 aacaaagaag tgcacaagtc tgggtacctc agctctgagc ggctgatccc tcaaagagtg
     1261 atggaccagc acaaacttac cagggaccag tgggaggacc ggatccaggt gtggcatgcg
     1321 gaacaccgtg ggatgctcaa agataatgct atgttggaat acctgaagat tgctcaggac
     1381 ctggaaatgt atggaatcaa ctatttcgag ataaaaaaca agaaaggaac agacctttgg
     1441 cttggagttg atgcccttgg actgaatatt tatgagaaag atgataagtt aaccccaaag
     1501 attggctttc cttggagtga aatcaggaac atctctttca atgacaaaaa gtttgtcatt
     1561 aaacccatcg acaagaaggc acctgacttt gtgttttatg ccccacgtct gagaatcaac
     1621 aagcggatcc tgcagctctg catgggcaac catgagttgt atatgcgccg caggaagcct
     1681 gacaccatcg aggtgcagca gatgaaggcc caggcccggg aggagaagca tcagaagcag
     1741 ctggagcggc aacagctgga aacagagaag aaaaggagag aaaccgtgga gagagagaaa
     1801 gagcagatga tgcgcgagaa ggaggagttg atgctgcggc tgcaggacta tgaggagaag
     1861 acaaagaagg cagagagaga gctctcggag cagattcaga gggccctgca gctggaggag
     1921 gagaggaagc gggcacagga ggaggccgag cgcctagagg ctgaccgtat ggctgcactg
     1981 cgggctaagg aggagctgga gagacaggcg gtggatcaga taaagagcca ggagcagctg
     2041 gctgcggagc ttgcagaata cactgccaag attgccctcc tggaagaggc gcggaggcgc
     2101 aaggaggatg aagttgaaga gtggcagcac agggccaaag aagcccagga tgacctggtg
     2161 aagaccaagg aggagctgca cctggtgatg acagcacccc cgcccccacc accccccgtg
     2221 tacgagccgg tgagctacca tgtccaggag agcttgcagg atgagggcgc agagcccacg
     2281 ggctacagcg cggagctgtc tagtgagggc atccgggatg accgcaatga ggagaagcgc
     2341 atcactgagg cagagaagaa cgagcgtgtg cagcggcagc tgctgacgct gagcagcgag
     2401 ctgtcccagg cccgagatga gaataagagg acccacaatg acatcatcca caacgagaac
     2461 atgaggcaag gccgggacaa gtacaagacg ctgcggcaga tccggcaggg caacaccaag
     2521 cagcgcatcg acgagttcga ggccctgtaa gaattctgca gtcgacggta ccgcgggccc
     2581 gggatccacc ggatctagat aactgatcat aatcagccat accacat
//
