LOCUS       sequence-84265-e        4001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4001)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-FAK-5
FEATURES             Location/Qualifiers
     source          1..4001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctatgg cagcagctta
      781 ccttgatcca aacttgaatc atacaccaag ttcaagtgca aagacgcacc tcggtactgg
      841 gatggagcgt tccccggggg ccatggagcg agtcctaaag gtttttcact actttgaaaa
      901 cagcagcgag ccaacgacgt gggccagcat tatccggcat ggagatgcta ctgatgttcg
      961 aggcataata cagaagattg tggactgtca caaagtgaaa aatgtggcct gctatgggtt
     1021 gcgactcagt catctgcagt ctgaggaggt tcactggctg cacctggaca tgggggtatc
     1081 caatgtgaga gagaaatttg aactagcaca tcctccagaa gaatggaaat atgaactgag
     1141 aattcggtac ctgcccaaag gatttctaaa ccagttcact gaggacaaac caactttaaa
     1201 ttttttctat cagcaggtga aaaatgacta tatgttagaa atagcagatc aagtggacca
     1261 ggaaattgct ttgaaactag gttgccttga aatcaggaga tcctacggag agatgagagg
     1321 caatgcatta gagaagaaat ccaactatga agtgctagaa aaagatgtcg gtttaagacg
     1381 attttttccg aagagtttgc tagattcagt gaaggccaaa acactacgaa aattaatcca
     1441 acagacattt cgacaatttg ccaacctcaa cagagaagaa agtattttga aattctttga
     1501 gatcctctct ccagtgtaca gatttgacaa ggaatgcttc aagtgtgccc ttggttcaag
     1561 ctggattatt tcagtggagc tggcaattgg cccagaggaa ggaatcagct accttacaga
     1621 caagggtgca aatccaactc acctggcaga ttttaatcaa gtacaaacta ttcagtattc
     1681 aaacagtgaa gacaaggaca gaaaagggat gttgcaactg aagatagctg gtgcacctga
     1741 gcctctgaca gtgacagcac catccttaac cattgcagag aatatggctg acttgataga
     1801 cggatactgc cgactggtga atggagccac gcaatctttt attatcaggc cacagaaaga
     1861 aggtgaaaga gctttaccat caataccaaa gctggccaac aatgagaagc aaggagtaag
     1921 gtcgcacaca gtctctgtat cagaaacaga tgactatgca gagataatag atgaagaaga
     1981 tacttataca atgccatcaa ccagagatta tgaaattcaa agggagagaa ttgaactggg
     2041 gcgctgcatt ggtgaaggac agtttggaga tgtgcaccaa ggaatttaca tgagtccgga
     2101 aaatccagct atggctgtag caatcaaaac atgtaaaaac tgcacctcag acagcgttag
     2161 agaaaagttc ctacaagaag ccttaacaat gcgtcagttt gatcatcctc acattgtgaa
     2221 gctcattgga gttattacag aaaacccagt gtggataatc atggagctct gtacacttgg
     2281 agagttgaga tcgtttctgc aagtaagaaa attcagcttg gacctggcct ccctcatcct
     2341 ctacgcttac cagcttagca cagcacttgc ttacctagag agcaaaagat ttgtacatag
     2401 agatattgct gctaggaacg tgctggtatc tgccactgac tgtgtgaaat tgggtgactt
     2461 tggcttatcc cgatacatgg aagacagtac ttactataaa gcttccaaag gaaagttacc
     2521 tatcaaatgg atggctccag agtcaatcaa cttccgacgg tttacctcag caagcgatgt
     2581 gtggatgttt ggtgtgtgta tgtgggagat cctgatgcat ggggtaaagc ccttccaggg
     2641 agtgaaaaat aatgatgtta ttggtcggat tgagaacggt gagcggctcc ccatgcctcc
     2701 gaactgccct cccaccctct acagccttat gaccaagtgc tgggcatacg accctagtag
     2761 acgacccagg tttactgaac ttaaagcaca actcagtaca atactggagg aggagaagct
     2821 gcagcaagag gaacgaatga gaatggaatc caggcgacaa gtcacagtat cctgggactc
     2881 aggaggatca gatgaagctc ctcccaagcc cagcaggcct ggttacccca gcccaaggtc
     2941 cagtgaaggg ttttatccga gtcctcagca tatggtacag ccaaatcact accaggtatc
     3001 tggctactct ggttctcatg ggataccagc catggcaggc agcatttatc ctgggcaagc
     3061 ttctctcttg gatcaaacag attcctggaa ccatcgacct caggaagtat cagcatggca
     3121 gccaaacatg gaggattcgg gcactttgga tgtacgagga atggggcagg ttctgcccac
     3181 acatctcatg gaggagaggt taataagaca acagcaagag atggaagaag atcaacgctg
     3241 gcttgagaaa gaggaacgat tcctggtaat gaaacctgat gtgcggctct ccagaggcag
     3301 cattgaacgg gaggacggag gtctccaggg cccagctggt aaccagcaca tatatcagcc
     3361 tgtgggtaaa ccagatcatg ccgctccacc aaagaagccc cctcgccctg gagcccccca
     3421 cttgggcagc ctcgcgagcc tgaacagccc cgtggacagc tacaacgaag gcgtgaagat
     3481 caagccacag gaaatcagcc ctcctcctac ggccaacctg gaccgctcca atgacaaagt
     3541 ctatgagaat gtaaccgggc tggtgaaagc tgtcatagag atgtccagta aaatacagcc
     3601 agctccgcca gaggagtacg tgcccatggt aaaggaggtt ggcttggcgc tgagaacctt
     3661 gctagcaaca gtggatgagt cgctgccagt gcttcctgca agcacccaca gagagattga
     3721 gatggcccag aaactgctga actctgacct ggctgagctc attaacaaga tgaagctggc
     3781 ccagcagtac gtcatgacca gcctgcagca ggagtacaag aagcaaatgc tgacggctgc
     3841 tcacgctctg gctgtggatg ccaagaactt gctggatgtc atcgatcaag ccagactgaa
     3901 aatgatcagc cagtccaggc ccactaagct gccgtagccg aattctgcag atgggatcca
     3961 ccggatctag ataactgatc ataatcagcc ataccacatt g
//
