LOCUS       sequence-84266-e        3506 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3506)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-FAM186B-N-18
FEATURES             Location/Qualifiers
     source          1..3506
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2751..2760
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2757..2759,2760..2762,2763..3476)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2802..2823)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3063..3082)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3410..3431
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggaga aggatgaccc cccacagttg gtgactccca
       61 catcagtgaa agccatcatc ctgaggattg aggctgccca gctaactcgg gctcaagagg
      121 atatttctac ccagctctca gacattttgg acaatgtcaa ttgtgtcatc aaccgcttcc
      181 aggaagaatt aggatatgat ttaaaagaaa atgccaaatc tcagcagaga gatccaaagg
      241 gcaagaagag attcatcttg ctggaaaaaa ttgcctcctt ctccaaagat gctatgatga
      301 aggagaagca cctgtatgac attctccgct ggctgggtga ctggggtgac actctgacct
      361 atgagattgg gcccaggaag agtgaagagg aagcagcagc tctggacgaa tggattgaag
      421 tgacggagaa agtgttaccg ctgtccctca ttgccaccaa aagaggcatc gagtcactca
      481 ctgccctttg ctccactctc attgaaggac aaaagaaaag gtcacaagtg tccaaacgca
      541 ccttctggca gggctggcag ggaagaagcc cacagacatc tccatcccat cctcagccac
      601 taagcccaga acagatgctc caggaccagc ataccatgaa cacgaaggcc tcggaggtga
      661 cgtccatgct gcaggagctc ctggactcta ccatgttcag caagggggag gtcagggcca
      721 tcaggtacat ggccactgtg gtggagaacc tcaacaaggc cttgatcctc caacacaagg
      781 agaacaggag cctggagacc aaatacaggc acctgcaaat gcaggcgacc aaagagctca
      841 gcagccagag gctgcacttc cagcagttca tggaggtcct tgagagcagg agggatgctc
      901 tgctgaagca ggtagagatc ttagggggaa ggtaccatga ccttctcctg atgaagcagg
      961 ccttggagtt ccagctgaag aaggctcaga atgctacagg tcaggcagaa gacctggctg
     1021 aggtttctgt tgactcccca ggtccctctg agagagagac cctcccaagg aaagaaacag
     1081 tcatggagga aagccaacag gaaccgatga aggaggagca gttgttctcg ccacttcccc
     1141 caagtcccat ggccatgata cgggacagtg gtgctatagc tgcagggcac cagccacttt
     1201 ccaccatgac tatgcgctcg agggtcgcag atgtgttcgg cagcaaggac actgagagcc
     1261 ttgagcctgt gcttttaccc ttagtagatc gcaggtttcc taagaaatgg gaaagaccgg
     1321 tggcagaaag cttaggccac aaagacaaag accaggagga ctacttccag aagggaggac
     1381 tccaaattaa gttccactgt agcaagcagc tgtctctaga gagctccagg caggtgacct
     1441 ctgagagcca agaggagccc tgggaggagg aattcggccg ggagatgcgg aggcagctgt
     1501 ggctggagga ggaggagatg tggcagcagc ggcagaagaa gtgggccctg ctggagcagg
     1561 agcatcagga gaagctgcgg cagtggaatc tggaagacct ggccagggag caacagcgga
     1621 gatgggtcca gctagaaaag gagcaggaga gcccacggag agagccagag cagctagggg
     1681 aggatgtgga gaggaggatc ttcacaccca ccagtcgatg gagggacttg gagaaggcag
     1741 agctatcatt agtgcctgcc ccaagccgga cccaatctgc tcaccaaagc aggaggccac
     1801 acttgcccat gtctcctagt acccagcagc ctgccctggg aaagcagaga cctatgagtt
     1861 cagtggagtt tacctacaga ccacggaccc gccgagttcc cacaaagccc aagaaatctg
     1921 cctcctttcc tgtcactggg acatccatcc gaaggctgac ctggccctct ttgcagatat
     1981 cccctgcaaa tattaagaag aaggtgtacc acatggacat ggaggcccag aggaagaacc
     2041 tgcagctcct gagtgaggag tctgagttga ggctgcccca ctacctgcgc agcaaagcac
     2101 tggagctcac caccaccacc atggagctgg gcgcgctcag gctgcagtac ctgtgccata
     2161 agtacatctt ctatagacgc ctccagagcc tccggcaaga agcgatcaac catgtacaaa
     2221 tcatgaaaga aacggaggct tcctacaagg cccagaacct ctacatcttc ctggaaaaca
     2281 ttgaccgcct gcagagtctc aggctgcagg cctggacgga caagcagaag gggctggagg
     2341 agaagcaccg agagtgcctg agcagcatgg tgaccatgtt ccccaagctc cagctggagt
     2401 ggaacgttca cctgaacatc cctgaggtca cctcgccaaa gccaaagaaa tgcaagttgc
     2461 ctgcagcctc accccggcac atccgcccca gtggccccac ctacaagcag ccctttctgt
     2521 ctaggcaccg ggcatgtgtg cccctgcaga tggcccgcca acaggggaag cagatggagg
     2581 ctgtctggaa gaccgaggtg gcctcctcca gttacgcaat agaaaaaaag acccctgcca
     2641 gccttccccg ggaccagctg aggggacacc cagatattcc ccggctgttg acactggacg
     2701 tgggcagcgg gagtggcagt cgcggaggca gtggtgggga tccaccggtc gccaccatgg
     2761 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2821 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2881 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2941 tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     3001 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3061 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3121 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3181 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
     3241 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3301 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3361 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3421 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     3481 ccgcgactct tagatcataa tcagcc
//
