LOCUS       sequence-84268-e        2303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling Protein, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2303)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Fascin-C-10
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(33..35,36..38,39..749)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(78..99)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(339..358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     686..707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
       61 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc
      241 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      481 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
      541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      721 tcactctcgg catggacgag ctgtacaagt ccggagctgg tagtggtgga agcgcaggaa
      781 ccgccaacgg cacagccgag gcggtgcaga tccagttcgg cctcatcaac tgcggcaaca
      841 agtacctgac ggccgaggcg ttcgggttca aggtgaacgc gtccgccagc agcctgaaga
      901 agaagcagat ctggacgctg gagcagcccc ctgacgaggc gggcagcgcg gccgtgtgcc
      961 tgcgcagcca cctgggccgc tacctggcgg cggacaagga cggcaacgtg acctgcgagc
     1021 gcgaggtgcc cggtcccgac tgccgtttcc tcatcgtggc gcacgacgac ggtcgctggt
     1081 cgctgcagtc cgaggcgcac cggcgctact tcggcggcac cgaggaccgc ctgtcctgct
     1141 tcgcgcagac ggtgtccccc gccgagaagt ggagcgtgca catcgccatg caccctcagg
     1201 tcaacatcta cagcgtcacc cgtaagcgct acgcgcacct gagcgcgcgg ccggccgacg
     1261 agatcgccgt ggaccgcgac gtgccctggg gcgtcgactc gctcatcacc ctcgccttcc
     1321 aggaccagcg ctacagcgtg cagaccgccg accaccgctt cctgcgccac gacgggcgcc
     1381 tggtggcgcg ccccgagccg gccactggct acacgctgga gttccgctcc ggcaaggtgg
     1441 ccttccgcga ctgcgagggc cgttacctgg cgccgtcggg gcccagcggc acgctcaagg
     1501 cgggcaaggc caccaaggtg ggcaaggacg agctctttgc tctggagcag agctgcgccc
     1561 aggtcgtgct gcaggcggcc aacgagagga acgtgtccac gcgccagggt atggacctgt
     1621 ctgccaatca ggacgaggag accgaccagg agaccttcca gctggagatc gaccgcgaca
     1681 ccaaaaagtg tgccttccgt acccacacgg gcaagtactg gacgctgacg gccaccgggg
     1741 gcgtgcagtc caccgcctcc agcaagaatg ccagctgcta ctttgacatc gagtggcgtg
     1801 accggcgcat cacactgagg gcgtccaatg gcaagtttgt gacctccaag aagaatgggc
     1861 agctggccgc ctcggtggag acggcagggg actcagagct cttcctcatg aagctcatca
     1921 accgccccat catcgtgttc cgcggggagc atggcttcat cggctgccgc aaggtcacgg
     1981 gcaccctgga cgccaaccgc tccagctatg acgtcttcca gctggagttc aacgatggcg
     2041 cctacaacat caaagactcc acaggcaaat actggacggt gggcagtgac tccgtggtca
     2101 ccagcagcgg cgacactcct gtggacttct tcttcgagtt ctgcgactat aacaaggtgg
     2161 ccatcaaggt gggcgggcgc tacctgaagg gcgaccacgc aggcgtcctg aaggcctcgg
     2221 cggaaaccgt ggaccccgcc tcgctctggg agtactaggg atccaccgga tctagataac
     2281 tgatcataat cagccatacc agc
//
