LOCUS       sequence-84269-e        2287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling Protein, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2287)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Fascin-N-10
FEATURES             Location/Qualifiers
     source          1..2287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1521..1530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1527..1529,1530..1532,1533..2246)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1572..1593)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1833..1852)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2180..2201
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgccaccatg accgccaacg gcacagccga ggcggtgcag atccagttcg
       61 gcctcatcaa ctgcggcaac aagtacctga cggccgaggc gttcgggttc aaggtgaacg
      121 cgtccgccag cagcctgaag aagaagcaga tctggacgct ggagcagccc cctgacgagg
      181 cgggcagcgc ggccgtgtgc ctgcgcagcc acctgggccg ctacctggcg gcggacaagg
      241 acggcaacgt gacctgcgag cgcgaggtgc ccggtcccga ctgccgtttc ctcatcgtgg
      301 cgcacgacga cggtcgctgg tcgctgcagt ccgaggcgca ccggcgctac ttcggcggca
      361 ccgaggaccg cctgtcctgc ttcgcgcaga cggtgtcccc cgccgagaag tggagcgtgc
      421 acatcgccat gcaccctcag gtcaacatct acagcgtcac ccgtaagcgc tacgcgcacc
      481 tgagcgcgcg gccggccgac gagatcgccg tggaccgcga cgtgccctgg ggcgtcgact
      541 cgctcatcac cctcgccttc caggaccagc gctacagcgt gcagaccgcc gaccaccgct
      601 tcctgcgcca cgacgggcgc ctggtggcgc gccccgagcc ggccactggc tacacgctgg
      661 agttccgctc cggcaaggtg gccttccgcg actgcgaggg ccgttacctg gcgccgtcgg
      721 ggcccagcgg cacgctcaag gcgggcaagg ccaccaaggt gggcaaggac gagctctttg
      781 ctctggagca gagctgcgcc caggtcgtgc tgcaggcggc caacgagagg aacgtgtcca
      841 cgcgccaggg tatggacctg tctgccaatc aggacgagga gaccgaccag gagaccttcc
      901 agctggagat cgaccgcgac accaaaaagt gtgccttccg tacccacacg ggcaagtact
      961 ggacgctgac ggccaccggg ggcgtgcagt ccaccgcctc cagcaagaat gccagctgct
     1021 actttgacat cgagtggcgt gaccggcgca tcacactgag ggcgtccaat ggcaagtttg
     1081 tgacctccaa gaagaatggg cagctggccg cctcggtgga gacagcaggg gactcagagc
     1141 tcttcctcat gaagctcatc aaccgcccca tcatcgtgtt ccgcggggag catggcttca
     1201 tcggctgccg caaggtcacg ggcaccctgg acgccaaccg ctccagctat gacgtcttcc
     1261 agctggagtt caacgatggc gcctacaaca tcaaagactc cacaggcaaa tactggacgg
     1321 tgggcagtga ctccgtggtc accagcagcg gcgacactcc tgtggacttc ttcttcgagt
     1381 tctgcgacta taacaaggtg gccatcaagg tgggcgggcg ctacctgaag ggcgaccacg
     1441 caggcgtcct gaaggcctcg gcggaaaccg tggaccccgc ctcgctctgg gagtacgcat
     1501 ccagcgggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1561 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1621 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1681 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
     1741 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1801 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1861 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1921 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
     1981 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
     2041 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2101 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     2161 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2221 tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     2281 acatttt
//
