LOCUS       sequence-84270-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1547..1558)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgaagcc
      781 aggattcagt ccccgtgggg gtggctttgg cggccgaggg ggctttggtg accgtggtgg
      841 tcgtggaggc cgagggggct ttggcggggg ccgaggccaa ggcggaggct ttagaggtcg
      901 tggacgagga ggaggtggag gcggcggcgg cggtggagga ggaggaagag gtggtggagg
      961 cttccatttt ggtggcaacc ggggtcgtgg tcggggagga aaaagaggaa accagtcggg
     1021 gaagaatgtg atggtggagc cgcatcggca tgagggtgtc ttcatttgtc ggggaaagga
     1081 agatgcactg gtcaccaaga acctggtccc tggggaatca gtttatggag agaagagagt
     1141 ctcgatttcg gaaggagatg acaaaattga gtaccgagcc tggaacccct tccgctccaa
     1201 gcttgcagca gcaatcctgg gtggtgtgga ccagatccac atcaaaccgg gggctaaggt
     1261 tctctacctc ggggctgcct cgggcaccac ggtctcccat gtctctgaca tcgttggtcc
     1321 ggatggtcta gtctatgcag tcgagttctc ccaccgctct ggccgtgacc tcattaactt
     1381 ggccaagaag aggaccaaca tcattcctgt gatcgaggat gctcgacacc cacacaaata
     1441 ccgcatgctc atcgcaatgg tggatgtgat ctttgctgat gtggcccagc cagaccagac
     1501 ccggattgtg gccctgaatg cccacacctt cctgcgtaat ggaggacact ttgtgatttc
     1561 cattaaggcc aactgcattg actccacagc ctcagccgag gccgtgtttg cctccgaagt
     1621 gaaaaagatg caacaggaga acatgaagcc gcaggagcag ttgacccttg agccatatga
     1681 aagagaccat gccgtggtcg tgggagtgta caggccaccc cccaaggtga agaactgagg
     1741 atccaccgga tctagataac tgatcataat cagcc
//
