LOCUS       sequence-84272-e        8727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8727)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-FilaminA-N-9
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7964..7973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7970..7972,7973..7975,7976..8689)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8015..8036)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8276..8295)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8623..8644
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgag tagctcccac tctcgggcgg gccagagcgc
       61 agcaggcgcg gctccgggcg gcggcgtcga cacgcgggac gccgagatgc cggccaccga
      121 gaaggacctg gcggaggacg cgccatggaa gaagatccag cagaacactt tcacgcgctg
      181 gtgcaacgag cacctgaagt gcgtgagcaa gcgcatcgcc aacctgcaga cggacctgag
      241 cgacgggctg cggcttatcg cgctgttgga ggtgctcagc cagaagaaga tgcaccgcaa
      301 gcacaaccag cggcccactt tccgccaaat gcagcttgag aacgtgtcgg tggcactcga
      361 gttcctggac cgcgagagca tcaaactggt gtccatcgac agcaaggcca tcgtggacgg
      421 gaacctgaag ctgatcctgg gcctcatctg gaccctgatc ctgcactact ccatctccat
      481 gcccatgtgg gacgaggagg aggatgagga ggccaagaag cagaccccca agcagaggct
      541 cctgggctgg atccagaaca agctgccgca gctgcccatc accaacttca gccgggactg
      601 gcagagcggc cgggccctgg gcgccctggt ggacagctgt gccccgggcc tgtgtcctga
      661 ctgggactct tgggacgcca gcaagcccgt taccaatgcg cgagaggcca tgcagcaggc
      721 ggatgactgg ctgggcatcc cccaggtgat cacccccgag gagattgtgg accccaacgt
      781 ggacgagcac tctgtcatga cctacctgtc ccagttcccc aaggccaagc tgaagccagg
      841 ggctcccttg cggcccaaac tgaacccgaa gaaagcccgt gcctacgggc caggcatcga
      901 gcccacaggc aacatggtga agaagcgggc agagttcact gtggagacca gaagtgctgg
      961 ccagggagag gtgctggtgt acgtggagga cccggccgga caccaggagg aggcaaaagt
     1021 gaccgccaat aacgacaaga accgcacctt ctccgtctgg tacgtccccg aggtgacggg
     1081 gactcataag gttactgtgc tctttgctgg ccagcacatc gccaagagcc ccttcgaggt
     1141 gtacgtggat aagtcacagg gtgacgccag caaagtgaca gcccaaggtc ccggcctgga
     1201 gcccagtggc aacatcgcca acaagaccac ctactttgag atctttacgg caggagctgg
     1261 cacgggcgag gtcgaggttg tgatccagga ccccatggga cagaagggca cggtagagcc
     1321 tcagctggag gcccggggcg acagcacata ccgctgcagc taccagccca ccatggaggg
     1381 cgtccacacc gtgcacgtca cgtttgccgg cgtgcccatc cctcgcagcc cctacactgt
     1441 cactgttggc caagcctgta acccgagtgc ctgccgggcg gttggccggg gcctccagcc
     1501 caagggtgtg cgggtgaagg agacagctga cttcaaggtg tacacaaagg gcgctggcag
     1561 tggggagctg aaggtcaccg tgaagggccc caagggagag gagcgcgtga agcagaagga
     1621 cctgggggat ggcgtgtatg gcttcgagta ttaccccatg gtccctggaa cctatatcgt
     1681 caccatcacg tggggtggtc agaacatcgg gcgcagtccc ttcgaagtga aggtgggcac
     1741 cgagtgtggc aatcagaagg tacgggcctg gggccctggg ctggagggcg gcgtcgttgg
     1801 caagtcagca gactttgtgg tggaggctat cggggacgac gtgggcacgc tgggcttctc
     1861 ggtggaaggg ccatcgcagg ctaagatcga atgtgacgac aagggcgacg gctcctgtga
     1921 tgtgcgctac tggccgcagg aggctggcga gtatgccgtt cacgtgctgt gcaacagcga
     1981 agacatccgc ctcagcccct tcatggctga catccgtgac gcgccccagg acttccaccc
     2041 agacagggtg aaggcacgtg ggcctggatt ggagaagaca ggtgtggccg tcaacaagcc
     2101 agcagagttc acagtggatg ccaagcacgg tggcaaggcc ccacttcggg tccaagtcca
     2161 ggacaatgaa ggctgccctg tggaggcgtt ggtcaaggac aacggcaatg gcacttacag
     2221 ctgctcctac gtgcccagga agccggtgaa gcacacagcc atggtgtcct ggggaggcgt
     2281 cagcatcccc aacagcccct tcagggtgaa tgtgggagct ggcagccacc ccaacaaggt
     2341 caaagtatac ggccccggag tagccaagac agggctcaag gcccacgagc ccacctactt
     2401 cactgtggac tgcgccgagg ctggccaggg ggacgtcagc atcggcatca agtgtgcccc
     2461 tggagtggta ggccccgccg aagctgacat cgacttcgac atcatccgca atgacaatga
     2521 caccttcacg gtcaagtaca cgccccgggg ggctggcagc tacaccatta tggtcctctt
     2581 tgctgaccag gccacgccca ccagccccat ccgagtcaag gtggagccct ctcatgacgc
     2641 cagtaaggtg aaggccgagg gccctggcct cagtcgcact ggtgtcgagc ttggcaagcc
     2701 cacccacttc acagtaaatg ccaaagctgc tggcaaaggc aagctggacg tccagttctc
     2761 aggactcacc aagggggatg cagtgcgaga tgtggacatc atcgaccacc atgacaacac
     2821 ctacacagtc aagtacacgc ctgtccagca gggtccagta ggcgtcaatg tcacttatgg
     2881 aggggatccc atccctaaga gccctttctc agtggcagta tctccaagcc tggacctcag
     2941 caagatcaag gtgtctggcc tgggagagaa ggtggacgtt ggcaaagacc aggagttcac
     3001 agtcaaatca aagggtgctg gtggtcaagg caaagtggca tccaagattg tgggcccctc
     3061 gggtgcagcg gtgccctgca aggtggagcc aggcctgggg gctgacaaca gtgtggtgcg
     3121 cttcctgccc cgtgaggaag ggccctatga ggtggaggtg acctatgacg gcgtgcccgt
     3181 gcctggcagc ccctttcctc tggaagctgt ggcccccacc aagcctagca aggtgaaggc
     3241 gtttgggccg gggctgcagg gaggcagtgc gggctccccc gcccgcttca ccatcgacac
     3301 caagggcgcc ggcacaggtg gcctgggcct gacggtggag ggcccctgtg aggcgcagct
     3361 cgagtgcttg gacaatgggg atggcacatg ttccgtgtcc tacgtgccca ccgagcccgg
     3421 ggactacaac atcaacatcc tcttcgctga cacccacatc cctggctccc cattcaaggc
     3481 ccacgtggtt ccctgctttg acgcatccaa agtcaagtgc tcaggccccg ggctggagcg
     3541 ggccaccgct ggggaggtgg gccaattcca agtggactgc tcgagcgcgg gcagcgcgga
     3601 gctgaccatt gagatctgct cggaggcggg gcttccggcc gaggtgtaca tccaggacca
     3661 cggtgatggc acgcacacca ttacctacat tcccctctgc cccggggcct acaccgtcac
     3721 catcaagtac ggcggccagc ccgtgcccaa cttccccagc aagctgcagg tggaacctgc
     3781 ggtggacact tccggtgtcc agtgctatgg gcctggtatt gagggccagg gtgtcttccg
     3841 tgaggccacc actgagttca gtgtggacgc ccgggctctg acacagaccg gagggccgca
     3901 cgtcaaggcc cgtgtggcca acccctcagg caacctgacg gagacctacg ttcaggaccg
     3961 tggcgatggc atgtacaaag tggagtacac gccttacgag gagggactgc actccgtgga
     4021 cgtgacctat gacggcagtc ccgtgcccag cagccccttc caggtgcccg tgaccgaggg
     4081 ctgcgacccc tcccgggtgc gtgtccacgg gccaggcatc caaagtggca ccaccaacaa
     4141 gcccaacaag ttcactgtgg agaccagggg agctggcacg ggcggcctgg gcctggctgt
     4201 agagggcccc tccgaggcca agatgtcctg catggataac aaggacggca gctgctcggt
     4261 cgagtacatc ccttatgagg ctggcaccta cagcctcaac gtcacctatg gtggccatca
     4321 agtgccaggc agtcctttca aggtccctgt gcatgatgtg acagatgcgt ccaaggtcaa
     4381 gtgctctggg cccggcctga gcccaggcat ggttcgtgcc aacctccctc agtccttcca
     4441 ggtggacaca agcaaggctg gtgtggcccc attgcaggtc aaagtgcaag ggcccaaagg
     4501 cctggtggag ccagtggacg tggtagacaa cgctgatggc acccagaccg tcaattatgt
     4561 gcccagccga gaagggccct acagcatctc agtactgtat ggagatgaag aggtaccccg
     4621 gagccccttc aaggtcaagg tgctgcctac tcatgatgcc agcaaggtga aggccagtgg
     4681 ccccgggctc aacaccactg gcgtgcctgc cagcctgccc gtggagttca ccatcgatgc
     4741 aaaggacgcc ggggagggcc tgctggctgt ccagatcacg gatcccgaag gcaagccgaa
     4801 gaagacacac atccaagaca accatgacgg cacgtataca gtggcctacg tgccagacgt
     4861 gacaggtcgc tacaccatcc tcatcaagta cggtggtgac gagatcccct tctccccgta
     4921 ccgcgtgcgt gccgtgccca ccggggacgc cagcaagtgc actgtcacag gtgctggcat
     4981 cggccccacc attcagattg gggaggagac ggtgatcact gtggacacta aggcggcagg
     5041 caaaggcaaa gtgacgtgca ccgtgtgcac gcctgatggc tcagaggtgg atgtggacgt
     5101 ggtggagaat gaggacggca ctttcgacat cttctacacg gccccccagc cgggcaaata
     5161 cgtcatctgt gtgcgctttg gtggcgagca cgtgcccaac agccccttcc aagtgacggc
     5221 tctggctggg gaccagccct cggtgcagcc ccctctacgg tctcagcagc tggccccaca
     5281 gtacacctac gcccagggcg gccagcagac ttgggccccg gagaggcccc tggtgggtgt
     5341 caatgggctg gatgtgacca gcctgaggcc ctttgacctt gtcatcccct tcaccatcaa
     5401 gaagggcgag atcacagggg aggttcggat gccctcaggc aaggtggcgc agcccaccat
     5461 cactgacaac aaagacggca ccgtgaccgt gcggtatgca cccagcgagg ctggcctgca
     5521 cgagatggac atccgctatg acaacatgca catcccagga agccccttgc agttctatgt
     5581 ggattacgtc aactgtggcc atgtcactgc ctatgggcct ggcctcaccc atggagtagt
     5641 gaacaagcct gccaccttca ccgtcaacac caaggatgca ggagaggggg gcctgtctct
     5701 ggccattgag ggcccgtcca aagcagaaat cagctgcact gacaaccagg atgggacatg
     5761 cagcgtgtcc tacctgcctg tgctgccggg ggactacagc attctagtca agtacaatga
     5821 acagcacgtc ccaggcagcc ccttcactgc ccgggtcaca ggtgacgact ccatgcgtat
     5881 gtcccaccta aaggtcggct ctgctgccga catccccatc aacatctcag agacggatct
     5941 cagcctgctg acggccactg tggtcccgcc ctcgggccgg gaggagccct gtttgctgaa
     6001 gcggctgcgt aatggccacg tggggatttc attcgtgccc aaggagacgg gggagcacct
     6061 ggtgcatgtg aagaaaaatg gccagcacgt ggccagcagc cccatcccgg tggtgatcag
     6121 ccagtcggaa attggggatg ccagtcgtgt tcgggtctct ggtcagggcc ttcacgaagg
     6181 ccacaccttt gagcctgcag agtttatcat tgatacccgc gatgcaggct atggtgggct
     6241 cagcctgtcc attgagggcc ccagcaaggt ggacatcaac acagaggacc tggaggacgg
     6301 gacgtgcagg gtcacctact gccccacaga gccaggcaac tacatcatca acatcaagtt
     6361 tgccgaccag cacgtgcctg gcagcccctt ctctgtgaag gtgacaggcg agggccgggt
     6421 gaaagagagc atcacccgca ggcgtcgggc tccttcagtg gccaacgttg gtagtcattg
     6481 tgacctcagc ctgaaaatcc ctgaaattag catccaggat atgacagccc aggtgaccag
     6541 cccatcgggc aagacccatg aggccgagat cgtggaaggg gagaaccaca cctactgcat
     6601 ccgctttgtt cccgctgaga tgggcacaca cacagtcagc gtcaagtaca agggccagca
     6661 cgtgcctggg agccccttcc agttcaccgt ggggccccta ggggaagggg gagcccacaa
     6721 ggtccgagct gggggccctg gcctggagag agctgaagct ggagtgccag ccgaattcag
     6781 tatctggacc cgggaagctg gtgctggagg cctggccatt gctgtcgagg gccccagcaa
     6841 ggctgagatc tcttttgagg accgcaagga cggctcctgt ggtgtggctt atgtggtcca
     6901 ggagccaggt gactacgaag tctcagtcaa gttcaacgag gaacacattc ccgacagccc
     6961 cttcgtggtg cctgtggctt ctccgtctgg cgacgcccgc cgcctcactg tttctagcct
     7021 tcaggagtca gggctaaagg tcaaccagcc agcctctttt gcagtcagcc tgaacggggc
     7081 caagggggcg atcgatgcca aggtgcacag cccctcagga gccctggagg agtgctatgt
     7141 cacagaaatt gaccaagata agtatgctgt gcgcttcatc cctcgggaga atggcgttta
     7201 cctgattgac gtcaagttca acggtaccca catccctgga agccccttca agatccgagt
     7261 tggggagcct gggcatggag gggacccagg cttggtgtct gcttacggag caggtctgga
     7321 aggcggtgtc acagggaacc cagctgagtt cgtcgtgaac acgagcaatg cgggagctgg
     7381 tgccctgtcg gtgaccattg acggcccctc caaggtgaag atggattgcc aggagtgccc
     7441 tgagggctac cgcgtcacct atacccccat ggcacctggc agctacctca tctccatcaa
     7501 gtacggcggc ccctaccaca ttgggggcag ccccttcaag gccaaagtca caggcccccg
     7561 tctcgtcagc aaccacagcc tccacgagac atcatcagtg tttgtagact ctctgaccaa
     7621 ggccacctgt gccccccagc atggggcccc gggtcctggg cctgctgacg ccagcaaggt
     7681 ggtggccaag ggcctggggc tgagcaaggc ctacgtaggc cagaagagca gcttcacagt
     7741 agactgcagc aaagcaggca acaacatgct gctggtgggg gttcatggcc caaggacccc
     7801 ctgcgaggag atcctggtga agcacgtggg cagccggctc tacagcgtgt cctacctgct
     7861 caaggacaag ggggagtaca cactggtggt caaatggggg cacgagcaca tcccaggcag
     7921 cccctaccgc gttgtggtgc ccagcggcag cgcctcaccg gtcgccacca tggtgagcaa
     7981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     8041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     8101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     8161 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     8221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     8281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     8341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     8401 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     8461 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     8521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     8581 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     8641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac
     8701 tctagatcat aatcagccat accacat
//
