LOCUS       sequence-84276-e        1987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin , Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1987)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Gamma-Actin2-N-18
FEATURES             Location/Qualifiers
     source          1..1987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1212..1221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1218..1220,1221..1223,1224..1937)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1263..1284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1524..1543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1871..1892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcattgctag tgacgtcaga tccgctagcg ccaccatgtg tgaagaagag accaccgccc
       61 ttgtgtgtga caatggctct ggcctgtgca aggcaggctt tgcaggagat gatgccccca
      121 gggctgtctt cccctccatt gtgggccgcc ctagacatca gggtgtgatg gtgggaatgg
      181 gccagaaaga cagctatgtg ggggacgagg ctcagagcaa gcgtgggatc ctaactctca
      241 agtaccctat tgaacatggc atcatcacca actgggatga catggagaag atctggcacc
      301 actccttcta caatgagctt cgagtagcac cagaagagca ccccaccctg ctcacagagg
      361 cccccctaaa ccccaaagca aacagggaga agatgaccca gatcatgttc gaaaccttca
      421 atgtccctgc catgtatgtt gctattcagg ctgtgctctc actctatgca tccggccgta
      481 ccacaggcat cgtcctggat tcgggggatg gcgtcaccca caatgtcccc atctatgagg
      541 gctatgcact gccccatgcc atcatgcgtc ttgacctggc tggacgggat ctcacagact
      601 acctcatgaa gattctcaca gaaagaggct attcctttgt gaccacagct gagagagaaa
      661 ttgtacgaga catcaaggag aagctgtgct atgtagccct ggatttcgag aatgagatgg
      721 ccacagcagc ttcatcttct tccctggaga aaagctacga gttgcctgat gggcaggtca
      781 tcactattgg caacgaacgc ttccgctgcc cggagaccct cttccagcct tccttcattg
      841 gcatggagtc agctggaatt catgaaacaa catacaattc catcatgaag tgtgacattg
      901 acatccgcaa agatttgtat gctaacaatg tcctctctgg gggcactacc atgtaccctg
      961 gcattgctga caggatgcag aaggaaatca cagccttggc tcccagcacc atgaagatca
     1021 agattatcgc tcctcctgag cggaagtact cagtctggat tggcggctcc atcctggcct
     1081 ctctctccac cttccagcaa atgtggatca gcaagccaga gtatgatgag gcagggccct
     1141 ccattgtcca caggaaatgc ttcagtggca gcagcggcgg ttccagtggc agccagagta
     1201 gcggaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1261 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1321 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1381 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct
     1441 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1501 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1561 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1621 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg
     1681 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg
     1741 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1801 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     1861 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1921 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac cacattgtag
     1981 agatgcc
//
