LOCUS       sequence-84278-e        2134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 487, Emission: 
            509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2134)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-GFAP-N-18
FEATURES             Location/Qualifiers
     source          1..2134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1370..1379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1376..1378,1379..1381,1382..2095)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1421..1442)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1682..1701)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2029..2050
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgga gaggagacgc atcacctccg ctgctcgccg
       61 ctcctacgtc tcctcagggg agatgatggt ggggggcctg gctcctggcc gccgtctggg
      121 tcctggcacc cgcctctccc tggctcgaat gccccctcca ctcccaaccc gggtggattt
      181 ctccctggct ggggcactca atgctggctt caaggagacc cgggccagtg agcgggcaga
      241 gatgatggag ctcaatgacc gctttgccag ctacatcgag aaggttcgct tcctggaaca
      301 gcaaaacaag gcgctggctg ctgagctgaa ccagctgcgg gccaaggagc ccaccaagct
      361 ggcagacgtc taccaggctg agctgcgaga gctgcggctg cggctcgatc aactcaccgc
      421 caacagcgcc cggctggagg ttgagaggga caatctggca caggacctgg ccactgtgag
      481 gcagaagctc caggatgaaa ccaacctgag gctggaagcc gagaacaacc tggctgccta
      541 tagacaggaa gcagatgaag ccaccctggc ccgtctggat ctggagagga agattgagtc
      601 gctggaggag gagatccggt tcttgaggaa gatccacgag gaggaggttc gggaactcca
      661 ggagcagctg gcccgacagc aggtccatgt ggagcttgac gtggccaagc cagacctcac
      721 cgcagccctg aaagagatcc gcacgcagta tgaggcaatg gcgtccagca acatgcatga
      781 agccgaagag tggtaccgct ccaagtttgc agacctgaca gacgctgctg cccgcaacgc
      841 ggagctgctc cgccaggcca agcacgaagc caacgactac cggcgccagt tgcagtcctt
      901 gacctgcgac ctggagtctc tgcgcggcac gaacgagtcc ctggagaggc agatgcgcga
      961 gcaggaggag cggcacgtgc gggaggcggc cagttatcag gaggcgctgg cgcggctgga
     1021 ggaagagggg cagagcctca aggacgagat ggcccgccac ttgcaggagt accaggacct
     1081 gctcaatgtc aagctggccc tggacatcga gatcgccacc tacaggaagc tgctagaggg
     1141 cgaggagaac cggatcacca ttcccgtgca gaccttctcc aacctgcaga ttcgagaaac
     1201 cagcctggac accaagtctg tgtcagaagg ccacctcaag aggaacatcg tggtgaagac
     1261 cgtggagatg cgggatggag aggtcattaa ggagtccaag caggagcaca aggatgtgat
     1321 gagcagcggt ggagcaagcg cagccagtgg tagcgcggat ccaccggtcg ccaccatggt
     1381 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1441 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1501 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1561 gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1621 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1681 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1741 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1801 ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat
     1861 caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1921 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1981 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2041 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc
     2101 cgcgactcta gatcataatc agccatacca catt
//
