LOCUS       sequence-84280-e        1390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1390)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H1-10
FEATURES             Location/Qualifiers
     source          1..1390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      631..640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(637..639,640..642,643..1356)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(682..703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(943..962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1290..1311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagccgc caccatgacc gagaactcca cctccgcccc ggcggcgaag
       61 cccaaacggg ccaaggcttc caagaagtcc acggaccacc ccaagtattc agacatgatc
      121 gtggctgcta tccaggcaga gaagaaccgt gccggctcct cgcgccagtc catccaaaag
      181 tatatcaaga gccactacaa ggtgggtgag aacgccgact cccagatcaa gttgtccatc
      241 aagcgcctag tgaccaccgg tgttctcaag caaaccaaag gggtgggcgc ctcggggtcc
      301 ttcaggctgg ccaagggcga tgagcccaaa aggtcggtgg ctttcaagaa gaccaagaag
      361 gaagtcaaga aagtggccac tccaaagaag gcagccaagc ccaagaaggc tgcctccaaa
      421 gccccaagca agaaacccaa agccacccct gtcaagaagg ccaagaagaa gccggctgcc
      481 acgcccaaga aagccaaaaa gcccaaggtt gtcaaagtca aaccagtcaa ggcctccaaa
      541 cccaagaagg ccaaaaccgt gaagcccaaa gccaagtcga gtgccaagag ggggagcaag
      601 aagaagtcgg gaagcacgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      661 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      721 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      781 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc
      841 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      901 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      961 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1021 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1081 cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc
     1141 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1201 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1261 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1321 gggatcactc tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
     1381 cagccatacc
//
