LOCUS       sequence-84281-e        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H2A-10
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      440..449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(446..448,449..451,452..1165)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(491..512)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(752..771)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1099..1120
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacgtcagat ccgctagccg ccaccatgtc gggacgtggc aagcagggcg gcaaagctcg
       61 cgccaaggcc aagacccgct cttctcgggc cgggcttcag tttcccgtag gccgagtgca
      121 tcgcctgctc cgcaaaggca actatgcgga gcgggtcggt gctggagcgc cggtgtacct
      181 ggcggcggtg ctggagtacc tgaccgccga gatcctggag ctggctggca acgcggcccg
      241 cgacaacaag aagactcgca tcatcccgcg tcacctccag ctggccatcc gcaacgatga
      301 ggagctcaac aagcttctgg gcaaagtcac catcgcacag ggtggcgtcc tgcccaacat
      361 ccaggccgtg ctactgccca agaagaccga gagccaccac aaggcgaagg gcaagtcggg
      421 aagcacggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      481 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      541 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      601 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt
      661 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      721 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      781 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      841 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
      901 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
      961 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1021 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     1081 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1141 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
     1201 cat
//
