LOCUS       sequence-84285-e        1217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1217)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H2B-N-22
FEATURES             Location/Qualifiers
     source          1..1217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..398
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     misc_feature    399..451
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(465..467,468..470,471..1184)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(510..531)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(771..790)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1118..1139
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg
       61 gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc
      121 gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg
      181 gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca
      241 tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg
      301 agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg
      361 agggtactaa ggccatcacc aagtacacca gcgctaagct cgagctcaag cttcgaattc
      421 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      481 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      541 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      601 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga
      661 cctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      721 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      781 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      841 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      901 acaacagcca caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact
      961 tcaagacccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1021 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1081 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1141 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     1201 atcataatca gccatac
//
