LOCUS       sequence-84287-e        1253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1253)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      489..498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(495..497,498..500,501..1214)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(540..561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(801..820)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1148..1169
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcgc caccatggcc cgaaccaagc
       61 agaccgctag gaagtccacc ggtgggaaag ccccccgcaa acagctggcc accaaggcgg
      121 ctcggaaaag cgcgccctct accggcgggg tgaagaagcc tcaccgctac aggccaggga
      181 ccgtggctct gagagagatc cgtcgttacc agaaatcgac tgagctgctc atccggaagc
      241 tgccattcca gagattggtg agggagatcg cccaggattt caaaaccgac ttgaggtttc
      301 aaagtgcagc catcggtgcc cttcaggagg ctagcgaagc atacctggtg gggttgtttg
      361 aagataccaa tctgtgtgcc atccacgcca agagagtcac catcatgccc aaagacatcc
      421 agttggctcg ccggatacgg ggggagagag ctgggtccgg aagcgcaggc agtggggatc
      481 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      541 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      601 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      661 tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc gcccgctacc
      721 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      781 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      841 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      901 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
      961 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     1021 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1081 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     1141 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1201 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac att
//
