LOCUS       sequence-84289-e        1928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Adaptor, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1928)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Homer1-C-18
FEATURES             Location/Qualifiers
     source          1..1928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(23..25,26..28,29..739)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(68..89)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(329..348)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     676..697
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
       61 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      121 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      181 gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc
      241 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      301 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      361 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      421 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
      481 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
      541 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      601 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
      661 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      721 catggacgag ctgtacaagt ccggagggag cggcagtggg tccagtggag ggagcagtgg
      781 aagcggaggg agcggggaac aacctatctt cagcactcga gctcatgtct tccagatcga
      841 cccaaacaca aagaagaact gggtacccac cagcaagcat gcagttactg tgtcttattt
      901 ctatgacagc acaaggaatg tgtataggat aatcagttta gacggctcaa aggcaataat
      961 aaatagcacc atcactccaa acatgacatt tactaaaaca tctcaaaagt ttggccaatg
     1021 ggctgatagc cgggcaaaca ctgtttatgg actgggattc tcctctgagc atcatctctc
     1081 aaaatttgca gaaaagtttc aggaatttaa agaagctgct cggctggcaa aggagaagtc
     1141 gcaggagaag atggaactga ccagtacccc ttcacaggaa tcagcaggag gagatcttca
     1201 gtctccttta acaccagaaa gtatcaatgg gacagatgat gagagaacac ccgatgtgac
     1261 acagaactca gagccaaggg ctgagccagc tcagaatgca ttgccatttt cacatagtgc
     1321 cggggatcga acccagggcc tctctcatgc tagttcagcc atcagcaaac actgggaggc
     1381 tgaactagcc acgctcaagg ggaacaatgc caagctcacc gcagcgctgc tggagtccac
     1441 tgccaacgtg aagcagtgga agcaacagct ggctgcctac caggaggagg cagagcggct
     1501 gcacaagcgg gtcacggagc tggaatgtgt tagtagtcaa gcaaacgcgg tgcacagcca
     1561 caagacagag ctgagtcaga cagtgcagga gctggaagag accctaaaag taaaggaaga
     1621 ggaaatagaa agattaaaac aagaaattga taacgccaga gaacttcaag aacagaggga
     1681 ctctttgact cagaaactac aggaagttga gattcgaaat aaagacctgg aggggcagct
     1741 gtcggagctg gagcagcgcc tggagaagag ccagagcgag caggacgctt tccgcagtaa
     1801 cctgaagact ctcctagaga ttctggacgg gaaaatattt gaactaacag aattgcggga
     1861 taatttggcc aagctactag aatgcagcta aggatccacc ggatctagat aactgatcat
     1921 aatcagcc
//
