LOCUS       sequence-84291-e        1384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Heterochromatin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1384)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-HP1-10
FEATURES             Location/Qualifiers
     source          1..1384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      623..632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(629..631,632..634,635..1348)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(674..695)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(935..954)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1282..1303
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggg aaagaaaacc aagcggacag ctgacagttc
       61 ttcttcagag gatgaggagg agtatgttgt ggagaaggtg ctagacaggc gcgtggttaa
      121 gggacaagtg gaatatctac tgaagtggaa aggcttttct gaggagcaca atacttggga
      181 acctgagaaa aacttggatt gccctgagct aatttctgaa tttatgaaaa agtataagaa
      241 gatgaaggag ggtgaaaata ataaacccag ggagaagtca gaaagtaaca agaggaaatc
      301 caatttctca aacagtgccg atgacatcaa atctaaaaaa aagagagagc agagcaatga
      361 tatcgctcgg ggctttgaga gaggactgga accagaaaag atcattgggg caacagattc
      421 ctgtggtgat ttaatgttcc taatgaaatg gaaagacaca gatgaagctg acctggttct
      481 tgcaaaagaa gctaatgtga aatgtccaca aattgtgata gcattttatg aagagagact
      541 gacatggcat gcatatcctg aggatgcgga aaacaaagag aaagaaacag caaagagctc
      601 gggaagcacg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      661 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      721 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      781 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
      841 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      901 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      961 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1021 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
     1081 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
     1141 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1201 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     1261 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1321 tctcggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata
     1381 ccac
//
