LOCUS       sequence-84293-e        2229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2229)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-ILK-C-14
FEATURES             Location/Qualifiers
     source          1..2229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(36..38,39..41,42..752)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(342..361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     689..710
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
       61 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      121 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      181 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
      241 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      301 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      361 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      421 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      481 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
      541 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      601 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
      661 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
      721 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga ggaagcggcg
      781 gatctgggag tggcgacgac attttcactc agtgccggga gggcaacgca gtcgccgttc
      841 gcctgtggct ggacaacacg gagaacgacc tcaaccaggg ggacgatcat ggcttctccc
      901 ccttgcactg ggcctgccga gagggccgct ctgctgtggt tgagatgttg atcatgcggg
      961 gggcacggat caatgtaatg aaccgtgggg atgacacccc cctgcatctg gcagccagtc
     1021 atggacaccg tgatattgta cagaagctat tgcagtacaa ggcagacatc aatgcagtga
     1081 atgaacatgg gaatgtgccc ctgcactatg cctgtttttg gggccaagat caagtggcag
     1141 aggacctggt ggcaaatggg gcccttgtca gcatctgtaa caagtatgga gagatgcctg
     1201 tggacaaagc caaggcaccc ctgagagagc ttctccgaga gcgggcagag aagatgggcc
     1261 agaatctcaa ccgtattcca tacaaggaca cattctggaa ggggaccacc cgcactcggc
     1321 cccgaaatgg aaccctgaac aaacactctg gcattgactt caaacagctt aacttcctga
     1381 cgaagctcaa cgagaatcac tctggagagc tatggaaggg ccgctggcag ggcaatgaca
     1441 ttgtcgtgaa ggtgctgaag gttcgagact ggagtacaag gaagagcagg gacttcaatg
     1501 aagagtgtcc ccggctcagg attttctcgc atccaaatgt gctcccagtg ctaggtgcct
     1561 gccagtctcc acctgctcct catcctactc tcatcacaca ctggatgcca tatggatccc
     1621 tctacaatgt actacatgaa ggcaccaatt tcgtcgtgga ccagagccag gctgtgaagt
     1681 ttgctttgga catggcaagg ggcatggcct tcctacacac actagagccc ctcatcccac
     1741 gacatgcact caatagccgt agtgtaatga ttgatgagga catgactgcc cgaattagca
     1801 tggctgatgt caagttctct ttccaatgtc ctggtcgcat gtatgcacct gcctgggtag
     1861 cccccgaagc tctgcagaag aagcctgaag acacaaacag acgctcagca gacatgtgga
     1921 gttttgcagt gcttctgtgg gaactggtga cacgggaggt accctttgct gacctctcca
     1981 atatggagat tggaatgaag gtggcattgg aaggccttcg gcctaccatc ccaccaggta
     2041 tttcccctca tgtgtgtaag ctcatgaaga tctgcatgaa tgaagaccct gcaaagcgac
     2101 ccaaatttga catgattgtg cctatccttg agaagatgca ggacaagtag gaattctgca
     2161 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
     2221 accacattt
//
