LOCUS       sequence-84297-e        3509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3509)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Inversin-C-18
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgcttag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
      541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagtccgga agtgctggca gcggtggagg cagcgcatcc
      781 ggcggaggca gcggaagcaa caagtcagag aacctgctgt ttgctggttc atcattagca
      841 tcacaagtcc atgctgctgc cgttaatgga gataagggtg ctctacagag gctcatcgta
      901 ggaaactctg ctcttaaaga caaagaagat cagtttggga gaacaccact tatgtattgc
      961 gtgttggctg acagattgga ttgtgcagat gctcttctga aggcaggagc agatgtgaat
     1021 aaaactgacc atagccagag aacagccctc catcttgcag cccagaaggg aaattatcgt
     1081 ttcatgaaac tcttacttac acgcagagca aactggatgc aaaaggatct ggaagagatg
     1141 actcctttgc acttgaccac ccggcacagg agccctaagt gtttggcact tctgctgaag
     1201 tttatggcac caggagaagt ggatacacag gataaaaaca agcaaacagc tctgcattgg
     1261 agtgcctact acaataaccc tgagcatgtg aagctgctca tcaagcatga ttctaacatt
     1321 gggattcctg atgttgaagg caagatccca cttcactggg cagccaacca taaagatcca
     1381 agtgctgttc acacagtgag atgcattctg gatgctgctc caacagagtc tttactgaac
     1441 tggcaagact acgagggtcg aactcctctt cactttgcag ttgctgatgg gaatgtgacc
     1501 gtggttgatg tcttgacctc atatgaaagc tgcaatataa cgtcttatga taacttattt
     1561 cgaaccccac tgcactgggc agctttatta ggccatgcac agattgtcca tctcctttta
     1621 gaaagaaata agtctggaac tatcccatct gacagccaag gagccacacc tttgcactat
     1681 gctgctcaga gtaactttgc tgaaacggtt aaagtgtttt taaaacatcc ttcagtgaaa
     1741 gatgattcag acctggaagg aagaacatcc tttatgtggg cagctggcaa aggcagtgat
     1801 gatgtcctta gaactatgct gagcttaaaa tcggacatag atattaacat ggctgacaaa
     1861 tatggaggta cagctttgca tgctgctgct ctttctggcc atgtcagcac cgtgaagtta
     1921 ttactggaaa ataatgctca agtagatgct actgatgtta tgaaacatac tccacttttc
     1981 cgagcctgtg agatgggaca caaagatgtg attcagacac tcattaaagg tggagcaagg
     2041 gtagatctag ttgaccaaga tggacattct cttctacatt gggcagcact gggaggaaat
     2101 gctgatgttt gccagatatt aatagaaaat aagatcaatc caaatgtcca ggattatgca
     2161 ggaagaaccc ctttgcagtg tgcagcatat ggaggctata tcaactgcat ggcagttctc
     2221 atggaaaaca atgcagaccc taacattcaa gacaaagagg gaagaacagc tttgcattgg
     2281 tcctgcaaca atggatacct tgatgccatt aaattactgc tagactttgc tgctttccct
     2341 aatcagatgg aaaacaatga agagagatac acaccccttg attatgcttt gcttggtgag
     2401 cgccatgaag tgatccagtt catgttggag cacggtgccc tgtccatcgc agccatacaa
     2461 gacatcgccg ccttcaaaat ccaagctgtc tacaaagggt acaaggtcag aaaagccttc
     2521 cgagacagga aaaatctcct catgaagcat gaacagttga gaaaagatgc tgctgccaaa
     2581 aagcgagagg aagaaaacaa acgaaaagag gcagaacagc aaaaaggaag gcggagccca
     2641 gattcctgca gaccccaggc ccttccctgt ctgcctagca cccaggatgt gcccagcagg
     2701 cagagccggg cccccagcaa gcagcctcct gctggcaacg tggcccaagg ccctgagcca
     2761 agagacagca gaggatctcc aggagggtct ctaggcggag ccctccagaa ggagcagcat
     2821 gtttcctcag atttgcaggg aacaaactcc agaaggccaa atgaaacagc cagagaacat
     2881 tctaaaggcc aatctgcttg tgtccacttc agacccaatg aaggcagtga tggaagcagg
     2941 catccaggag ttccctctgt tgagaagtcc agagagctcc gactgcagat aattcagaga
     3001 gaacgaagga ggaaggagct gtttcgcaaa aagaacaagg cagcagcagt catccagcgc
     3061 gcctggcgaa gctaccagct caggaagcac ctgtcccacc ttcggcatat gaagcagctt
     3121 ggagctggag atgtggacag atggaggcaa gagtctacag cattgctcct ccaggtttgg
     3181 aggaaggaac tggaactaaa attcccccaa accactgcag taagcaaggc ccccaagagt
     3241 ccatccaagg gcacctcagg cacaaagtcc accaagcact cagtgcttaa gcaaatctat
     3301 ggttgttctc acgaagggaa aatacatcat cctacaagat ctgtaaaagc ctcttctgtg
     3361 ctgcgtctca actcagtgag caacctacag tgtatacatc tccttgagaa cagtggaaga
     3421 tcaaagaact tttcttataa cctgcaatca gctactcagc caaaaaacaa aacaaaacct
     3481 tgaggatcca ccggatctag ataactgat
//
