LOCUS       sequence-84298-e        3071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3071)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-JUP-C-14
FEATURES             Location/Qualifiers
     source          1..3071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(340..359)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      481 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
      541 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggaagtg ctgggagcgc aagcggaagc
      781 ggaggctccg gtgaggtgat gaacctgatg gagcagccta tcaaggtgac tgagtggcag
      841 cagacataca cctacgactc gggtatccac tcgggcgcca acacctgcgt gccctccgtc
      901 agcagcaagg gcatcatgga ggaggatgag gcctgcgggc gccagtacac gctcaagaaa
      961 accaccactt acacccaggg ggtgcccccc agccaaggtg acctggagta ccagatgtcc
     1021 acaacagcca gggccaaacg ggtgcgggag gccatgtgcc ctggtgtgtc aggcgaggac
     1081 agctcgcttc tgctggccac ccaggtggag gggcaggcca ccaacctgca gcgactggcc
     1141 gagccgtccc agctgctcaa gtcggccatt gtgcatctca tcaactacca ggacgatgcc
     1201 gagctggcca ctcgcgccct gcccgagctc accaaactgc tcaacgacga ggacccggtg
     1261 gtggtgacca aggcggccat gattgtgaac cagctgtcga agaaggaggc gtcgcggcgg
     1321 gccctgatgg gctcgcccca gctggtggcc gctgtcgtgc gtaccatgca gaataccagc
     1381 gacctggaca cagcccgctg caccaccagc atcctgcaca acctctccca ccaccgggag
     1441 gggctgctcg ccatcttcaa gtcgggtggc atccctgctc tggtccgcat gctcagctcc
     1501 cctgtggagt cggtcctgtt ctatgccatc accacgctgc acaacctgct cctgtaccag
     1561 gagggcgcca agatggccgt gcgcctggcc gacgggctgc aaaagatggt gcccctgctc
     1621 aacaagaaca accccaagtt cctggccatc accaccgact gcctgcagct cctggcctac
     1681 ggcaaccagg agagcaagct gatcatcctg gccaatggtg ggccccaggc cctcgtgcag
     1741 atcatgcgta actacagtta tgaaaagctg ctctggacca ccagtcgtgt gctcaaggtg
     1801 ctatccgtgt gtcccagcaa taagcctgcc attgtggagg ctggtgggat gcaggccctg
     1861 ggcaagcacc tgaccagcaa cagcccccgc ctggtgcaga actgcctgtg gaccctgcgc
     1921 aacctctcag atgtggccac caagcaggag ggcctggaga gtgtgctgaa gattctggtg
     1981 aatcagctga gtgtggatga cgtcaacgtc ctcacctgtg ccacgggcac actctccaac
     2041 ctgacatgca acaacagcaa gaacaagacg ctggtgacac agaacagcgg tgtggaggct
     2101 ctcatccatg ccatcctgcg tgctggtgac aaggacgaca tcacggagcc tgccgtctgc
     2161 gctctgcgcc acctcactag ccgccaccct gaggccgaga tggcccagaa ctctgtgcgt
     2221 ctcaactatg gcatcccagc catcgtgaag ctgctcaacc agcccaacca gtggccactg
     2281 gtcaaggcaa ccatcggctt gatcaggaat ctggccctgt gcccagccaa ccatgccccg
     2341 ctgcaggagg cagcggtcat cccccgcctc gtccaactgc tggtgaaggc ccaccaggat
     2401 gcccagcgcc acgtagctgc aggcacacag cagccctaca cggatggtgt gaggatggag
     2461 gagattgtgg agggctgcac cggagcactg cacatcctcg cccgggaccc catgaaccgc
     2521 atggagatct tccggctcaa caccattccc ctgtttgtgc agctcctgta ctcgtcggtg
     2581 gagaacatcc agcgcgtggc tgccggggtg ctgtgtgagc tggcccagga caaggaggcg
     2641 gccgacgcca ttgatgcaga gggggcctcg gccccactca tggagttgct gcactcccgc
     2701 aacgagggca ctgccaccta cgctgctgcc gtcctgttcc gcatctccga ggacaagaac
     2761 ccagactacc ggaagcgcgt gtccgtggag ctcaccaact ccctcttcaa gcatgacccg
     2821 gctgcctggg aggctgccca gagcatgatt cccatcaatg agccctatgg agatgacttg
     2881 gatgccacct accgccccat gtactccagc gatgtgcccc ttgacccgct ggagatgcac
     2941 atggacatgg atggagacta ccccatcgac acctacagcg acggcctcag gcccccgtac
     3001 cccactgcag accacatgct ggcctaggga tccaccggat ctagataact gatcataatc
     3061 agccatacca c
//
