LOCUS       sequence-84299-e        3078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3078)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-JUP-N-14
FEATURES             Location/Qualifiers
     source          1..3078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2317..2326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2323..2325,2326..2328,2329..3042)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2368..2389)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2629..2648)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2976..2997
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatgga ggtgatgaac
       61 ctgatggagc agcctatcaa ggtgactgag tggcagcaga catacaccta cgactcgggt
      121 atccactcgg gcgccaacac ctgcgtgccc tccgtcagca gcaagggcat catggaggag
      181 gatgaggcct gcgggcgcca gtacacgctc aagaaaacca ccacttacac ccagggggtg
      241 ccccccagcc aaggtgacct ggagtaccag atgtccacaa cagccagggc caaacgggtg
      301 cgggaggcca tgtgccctgg tgtgtcaggc gaggacagct cgcttctgct ggccacccag
      361 gtggaggggc aggccaccaa cctgcagcga ctggccgagc cgtcccagct gctcaagtcg
      421 gccattgtgc atctcatcaa ctaccaggac gatgccgagc tggccactcg cgccctgccc
      481 gagctcacca aactgctcaa cgacgaggac ccggtggtgg tgaccaaggc ggccatgatt
      541 gtgaaccagc tgtcgaagaa ggaggcgtcg cggcgggccc tgatgggctc gccccagctg
      601 gtggccgctg tcgtgcgtac catgcagaat accagcgacc tggacacagc ccgctgcacc
      661 accagcatcc tgcacaacct ctcccaccac cgggaggggc tgctcgccat cttcaagtcg
      721 ggtggcatcc ctgctctggt ccgcatgctc agctcccctg tggagtcggt cctgttctat
      781 gccatcacca cgctgcacaa cctgctcctg taccaggagg gcgccaagat ggccgtgcgc
      841 ctggccgacg ggctgcaaaa gatggtgccc ctgctcaaca agaacaaccc caagttcctg
      901 gccatcacca ccgactgcct gcagctcctg gcctacggca accaggagag caagctgatc
      961 atcctggcca atggtgggcc ccaggccctc gtgcagatca tgcgtaacta cagttatgaa
     1021 aagctgctct ggaccaccag tcgtgtgctc aaggtgctat ccgtgtgtcc cagcaataag
     1081 cctgccattg tggaggctgg tgggatgcag gccctgggca agcacctgac cagcaacagc
     1141 ccccgcctgg tgcagaactg cctgtggacc ctgcgcaacc tctcagatgt ggccaccaag
     1201 caggagggcc tggagagtgt gctgaagatt ctggtgaatc agctgagtgt ggatgacgtc
     1261 aacgtcctca cctgtgccac gggcacactc tccaacctga catgcaacaa cagcaagaac
     1321 aagacgctgg tgacacagaa cagcggtgtg gaggctctca tccatgccat cctgcgtgct
     1381 ggtgacaagg acgacatcac ggagcctgcc gtctgcgctc tgcgccacct cactagccgc
     1441 caccctgagg ccgagatggc ccagaactct gtgcgtctca actatggcat cccagccatc
     1501 gtgaagctgc tcaaccagcc caaccagtgg ccactggtca aggcaaccat cggcttgatc
     1561 aggaatctgg ccctgtgccc agccaaccat gccccgctgc aggaggcagc ggtcatcccc
     1621 cgcctcgtcc aactgctggt gaaggcccac caggatgccc agcgccacgt agctgcaggc
     1681 acacagcagc cctacacgga tggtgtgagg atggaggaga ttgtggaggg ctgcaccgga
     1741 gcactgcaca tcctcgcccg ggaccccatg aaccgcatgg agatcttccg gctcaacacc
     1801 attcccctgt ttgtgcagct cctgtactcg tcggtggaga acatccagcg cgtggctgcc
     1861 ggggtgctgt gtgagctggc ccaggacaag gaggcggccg acgccattga tgcagagggg
     1921 gcctcggccc cactcatgga gttgctgcac tcccgcaacg agggcactgc cacctacgct
     1981 gctgccgtcc tgttccgcat ctccgaggac aagaacccag actaccggaa gcgcgtgtcc
     2041 gtggagctca ccaactccct cttcaagcat gacccggctg cctgggaggc tgcccagagc
     2101 atgattccca tcaatgagcc ctatggagat gacttggatg ccacctaccg ccccatgtac
     2161 tccagcgatg tgccccttga cccgctggag atgcacatgg acatggatgg agactacccc
     2221 atcgacacct acagcgacgg cctcaggccc ccgtacccca ctgcagacca catgctggcc
     2281 gggggtgcgt caggcggcag cggatctgga ccggtcgcca ccatggtgag caagggcgag
     2341 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2401 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2461 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc
     2521 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     2581 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2641 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2701 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2761 aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc
     2821 aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2881 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2941 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3001 gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc gactctagat
     3061 cataatcagc cataccac
//
